[骨髓增生异常综合征患者外周血中自然杀伤细胞的亚型及功能生物标志物变化]

[Subtype and Functional Biomarker Changes of NK Cells in Peripheral Blood of Patients with Myelodysplastic Syndrome].

作者信息

Xu Shu-Juan, Shao Zong-Hong, Fu Rong, Wang Hua-Quan, Liu Hui, Liu Chun-Yan, Zhang Wei

机构信息

Department of Hematology, General Hospitnl of Tianjin Medical University, Tianjin 300052, China.

Department of Hematology, General Hospitnl of Tianjin Medical University, Tianjin 300052, China. E-mail:

出版信息

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2017 Jun;25(3):832-836. doi: 10.7534/j.issn.1009-2137.2017.03.036.

DOI:10.7534/j.issn.1009-2137.2017.03.036

PMID:28641645

Abstract

OBJECTIVE

To analyze the subtype and functional biomarker expression changes of natural kill cells(NK) in peripheral blood of patients with myelodysplastic syndrome(MDS) and normal people, so as to evaluate the relationships between these changes and hematopoietic functions and to explore the role of NK cells in the pathogenesis of MDS.

METHODS

The quantity of NK cells and the expression of biomarkers(NKp30,NKp46,NKG2A) on NK cells were detected by flow cytometry in 35 MDS patients from 2015 to 2016 in our hospital and 34 normal controls. The correlation between these changes and hematopoietic functions, including the percentages of neutrophil(ANC), hemoglobin in peripheral blood and the hematopoietic function in bone marrow(CD34%) were evaluated.

RESULTS

The percentage and quantity of NK cells and CD56 NK cells in MDS patients were significantly lower than those in normal controls(P<0.05); the percentage of CD56 NK cells was higher than that in controls. The percentage of CD56 NK cells in NK cells of MDS patients was significantly lower than that of controls; the percentage of CD56 NK cells in NK cells of MDS patients was significantly higher than that of controls. The expression of NKp30 and NKp46 of MDS patients was significantly lower than that of controls. In MDS group, the percentage of NK cells and CD56 NK cells of peripheral blood lymphocytes in high risk MDS group was significantly lower than that in low risk MDS group. The percentage of NK and CD56 NK cells negatively correlated with that of CD34% in bone marrow, but positively correlated with ANC and Hb. The CD34% in bone marrow negatively correlated with expression of NKp46, but positively correlated with expression of NKG2A.

CONCLUSION

The decrease of NK number and function may cause the immune surveillance and lead to disease progression.

摘要

目的

分析骨髓增生异常综合征（MDS）患者与正常人外周血中自然杀伤细胞（NK）的亚型及功能生物标志物表达变化，以评估这些变化与造血功能的关系，并探讨NK细胞在MDS发病机制中的作用。

方法

采用流式细胞术检测2015年至2016年我院35例MDS患者及34例正常对照者外周血中NK细胞数量及NK细胞表面生物标志物（NKp30、NKp46、NKG2A）的表达。评估这些变化与造血功能的相关性，包括外周血中性粒细胞百分比（ANC）、血红蛋白及骨髓造血功能（CD34%）。

结果

MDS患者NK细胞及CD56⁺NK细胞的百分比和数量均显著低于正常对照（P<0.05）；CD56brightNK细胞百分比高于对照。MDS患者NK细胞中CD56dimNK细胞百分比显著低于对照；MDS患者NK细胞中CD56brightNK细胞百分比显著高于对照。MDS患者NKp30和NKp46的表达显著低于对照。MDS组中，高危MDS组外周血淋巴细胞中NK细胞及CD56⁺NK细胞百分比显著低于低危MDS组。NK及CD56⁺NK细胞百分比与骨髓中CD34%呈负相关，但与ANC及Hb呈正相关。骨髓中CD34%与NKp46表达呈负相关，但与NKG2A表达呈正相关。