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梨形四膜虫-NT1中随着培养温度改变脂质流动性和脂肪酸组成的变化。

Changes in lipid fluidity and fatty acid composition with altered culture temperature in Tetrahymena pyriformis-NT1.

作者信息

Connolly J G, Brown I D, Lee A G, Kerkut G A

出版信息

Comp Biochem Physiol A Comp Physiol. 1985;81(2):287-92. doi: 10.1016/0300-9629(85)90137-9.

DOI:10.1016/0300-9629(85)90137-9
PMID:2864170
Abstract

Cultures of T. pyriformis-NT1 were grown at 20 degrees C (Tg 20 degrees C) and 38 degrees C (Tg 38 degrees C). G.L.C. analysis and D.P.H. fluorescence polarization measurements in extracted phospholipids indicated that there was increased saturation of fatty acids and relatively reduced fluidity as growth temperature was increased. Breakpoints occurred in the Arrhenius plots of fluorescence polarization at 16 degrees C for Tg 38 degrees C total extracted phospholipids and 9 degrees C for Tg 20 degrees C lipids.

摘要

梨形四膜虫-NT1在20℃（Tg 20℃）和38℃（Tg 38℃）下培养。气相色谱分析和对提取的磷脂进行的二苯基己三烯荧光偏振测量表明，随着生长温度升高，脂肪酸饱和度增加，流动性相对降低。对于Tg 38℃总提取磷脂，荧光偏振的阿累尼乌斯图在16℃出现断点；对于Tg 20℃脂质，在9℃出现断点。

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