Sharma Divakar, Bisht Deepa
Department of Biochemistry, National JALMA Institute for Leprosy and Other Mycobacterial DiseasesAgra, India.
Front Cell Infect Microbiol. 2017 Jun 8;7:240. doi: 10.3389/fcimb.2017.00240. eCollection 2017.
Tuberculosis is caused by , one of the most successful and deadliest human pathogen. Aminoglycosides resistance leads to emergence of extremely drug resistant strains of . Iron is crucial for the biological functions of the cells. Iron assimilation, storage and their utilization is not only involved in pathogenesis but also in emergence of drug resistance strains. We previously reported that iron storing proteins (bacterioferritin and ferritin) were found to be overexpressed in aminoglycosides resistant isolates. In this study we performed the STRING analysis of bacterioferritin & ferritin proteins and predicted their interactive partners [ferrochelatase (hemH), Rv1877 (hypothetical protein/probable conserved integral membrane protein), uroporphyrinogen decarboxylase (hemE) trigger factor (tig), transcriptional regulatory protein (MT3948), hypothetical protein (MT1928), glnA3 (glutamine synthetase), molecular chaperone GroEL (groEL1 & hsp65), and hypothetical protein (MT3947)]. We suggested that interactive partners of bacterioferritin and ferritin are directly or indirectly involved in growth, homeostasis, iron assimilation, virulence, resistance, and stresses.
结核病由结核分枝杆菌引起，它是最成功且最致命的人类病原体之一。氨基糖苷类耐药性导致出现结核分枝杆菌的极端耐药菌株。铁对细胞的生物学功能至关重要。铁的同化、储存及其利用不仅与发病机制有关，还与耐药菌株的出现有关。我们之前报道过，在对氨基糖苷类耐药的分离株中发现铁储存蛋白（细菌铁蛋白和铁蛋白）过表达。在本研究中，我们对细菌铁蛋白和铁蛋白进行了STRING分析，并预测了它们的相互作用伙伴[亚铁螯合酶（hemH）、Rv1877（假定蛋白/可能的保守整合膜蛋白）、尿卟啉原脱羧酶（hemE）触发因子（tig）、转录调节蛋白（MT3948）、假定蛋白（MT1928）、谷氨酰胺合成酶（glnA3）、分子伴侣GroEL（groEL1和hsp65）以及假定蛋白（MT3947）]。我们认为细菌铁蛋白和铁蛋白的相互作用伙伴直接或间接参与结核分枝杆菌的生长、稳态、铁同化、毒力、耐药性及应激反应。
