青鳉的基因组编辑

Genome Editing of Medaka.

作者信息

Ansai Satoshi, Kinoshita Masato

机构信息

Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake-cho, Sakyo-ku, Kyoto, 606-8502, Kyoto, Japan.

Division of Ecological Genetics, Department of Population Genetics, National Institute of Genetics, Yata 1111, Mishima, 411-8540, Shizuoka, Japan.

出版信息

Methods Mol Biol. 2017;1630:175-188. doi: 10.1007/978-1-4939-7128-2_15.

DOI:10.1007/978-1-4939-7128-2_15

PMID:28643259

Abstract

Medaka (Oryzias latipes), along with zebrafish (Danio rerio), is a useful experimental model fish. Here, we describe a simple method for generating medaka gene knock-out strains using an automated microchip electrophoresis system. We also describe a method for targeted gene knock-in using a plasmid carrying a sequence that does not cause off-target effects in medaka.

摘要

青鳉（Oryzias latipes）与斑马鱼（Danio rerio）一样，是一种有用的实验模式鱼类。在此，我们描述了一种使用自动微芯片电泳系统生成青鳉基因敲除品系的简单方法。我们还描述了一种使用携带在青鳉中不会产生脱靶效应序列的质粒进行靶向基因敲入的方法。