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乳酸发酵短杆菌赖氨酸途径相关基因在大肠杆菌中的克隆与表达

Cloning and expression in Escherichia coli of genes involved in the lysine pathway of Brevibacterium lactofermentum.

作者信息

Márquez G, Sousa J M, Sánchez F

出版信息

J Bacteriol. 1985 Oct;164(1):379-83. doi: 10.1128/jb.164.1.379-383.1985.

DOI:10.1128/jb.164.1.379-383.1985
PMID:2864331
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC214254/
Abstract

The Brevibacterium lactofermentum genes which complement Escherichia coli lysA and asd-1 mutants were identified, respectively, as a 1.9-kilobase PstI-ClaI fragment and a 2.5-kilobase PstI fragment by cloning into pBR325. Southern blot transfers show hybridization to chromosomal fragments of identical size. The putative B. lactofermentum asd and lysA products are 44 and 48 kilodaltons, respectively.

摘要

通过克隆到pBR325中，分别鉴定出了与大肠杆菌lysA和asd - 1突变体互补的乳酸发酵短杆菌基因，分别为一个1.9千碱基对的PstI-ClaI片段和一个2.5千碱基对的PstI片段。Southern印迹转移显示与相同大小的染色体片段杂交。推测的乳酸发酵短杆菌asd和lysA产物分别为44千道尔顿和48千道尔顿。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6806/214254/07edab9cd41b/jbacter00215-0392-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6806/214254/3c530b5ba99f/jbacter00215-0391-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6806/214254/c7dd451fc548/jbacter00215-0391-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6806/214254/5a0e0f1a77c9/jbacter00215-0392-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6806/214254/07edab9cd41b/jbacter00215-0392-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6806/214254/3c530b5ba99f/jbacter00215-0391-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6806/214254/c7dd451fc548/jbacter00215-0391-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6806/214254/5a0e0f1a77c9/jbacter00215-0392-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6806/214254/07edab9cd41b/jbacter00215-0392-b.jpg

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