Alp Ebru, Yilmaz Akin, Onen Hacer Ilke, Menevse Emine Sevda
Department of Medical Biology, Faculty of Medicine, Giresun University, Giresun, Turkey.
Department of Medical Biology, Faculty of Medicine, Hitit University, Corum, Turkey.
J Cancer Res Ther. 2017 Apr-Jun;13(2):346-350. doi: 10.4103/0973-1482.176172.
Cancer cells exert differential responses to chemotherapeutics and inhibitors. To the best of our knowledge, a few or no research has been performed until now to determine the effect of EF-24 and RAD001 on MDA-MB-231 breast cancer cells with regard to mRNA expression of apoptotic and anti-apoptotic genes.
In this study, we aimed to investigate the mRNA expression levels of apoptotic (caspase 2 [CASP2], CASP8, and CASP9) and anti-apoptotic (B-cell lymphoma 2 [BCL2] and BCL2-like protein 1 [BCL2L1]) genes after exposure to paclitaxel, EF-24, and RAD001 in MDA-MB-231 cells.
After treatment, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was used to measure cell viability. mRNA expressions were analyzed using quantitative real-time polymerase chain reaction.
Decrease in cell viability ratios was seen in a dose-dependent manner for all chemicals. MDA-MB-231 cells responded slightly different to paclitaxel, EF-24, and RAD001 at the transcriptional level of apoptotic and anti-apoptotic genes.
Our results showed that response of these cells to paclitaxel, EF-24, and RAD001 was found different at the transcriptional level of apoptotic and antiapoptotic genes. Therefore, understanding transcriptional changes after these drug exposure may give us a change to figure out more realistic results of the apoptotic pathway inhibition.
癌细胞对化疗药物和抑制剂有不同的反应。据我们所知，迄今为止，尚未进行过少量或没有研究来确定EF-24和RAD001对MDA-MB-231乳腺癌细胞凋亡和抗凋亡基因mRNA表达的影响。
在本研究中，我们旨在研究MDA-MB-231细胞暴露于紫杉醇、EF-24和RAD001后凋亡基因（半胱天冬酶2 [CASP2]、CASP8和CASP9）和抗凋亡基因（B细胞淋巴瘤2 [BCL2]和B细胞淋巴瘤2样蛋白1 [BCL2L1]）的mRNA表达水平。
处理后，采用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐法测定细胞活力。使用定量实时聚合酶链反应分析mRNA表达。
所有化学物质均呈剂量依赖性地降低细胞活力比率。MDA-MB-231细胞在凋亡和抗凋亡基因的转录水平上对紫杉醇、EF-24和RAD001的反应略有不同。
我们的结果表明，这些细胞在凋亡和抗凋亡基因的转录水平上对紫杉醇、EF-24和RAD001的反应不同。因此，了解这些药物暴露后的转录变化可能使我们有机会弄清楚凋亡途径抑制的更实际结果。
