Drobot Kateryna O, Matvieieva Nadiia A, Ostapchuk Andriy M, Kharkhota Maxim A, Duplij Volodymyr P
a Institute of Cell Biology and Genetic Engineering , National Academy of Sciences of Ukraine , Kyiv , Ukraine.
b D.K. Zabolotny Institute of Microbiology and Virology , National Academy of Sciences of Ukraine , Kyiv , Ukraine.
Prep Biochem Biotechnol. 2017 Sep 14;47(8):776-781. doi: 10.1080/10826068.2017.1342262. Epub 2017 Jun 23.
We studied the effect of genetic transformation on biologically active compound (artemisinin and its co-products (ART) as well as sugars) accumulation in Artemisia vulgaris and Artemisia dracunculus "hairy" root cultures. Glucose, fructose, sucrose, and mannitol were accumulated in A. vulgaris and A. dracunculus "hairy" root lines. Genetic transformation has led in some cases to the sugar content increasing or appearing of nonrelevant for the control plant carbohydrates. Sucrose content was 1.6 times higher in A. vulgaris "hairy" root lines. Fructose content was found to be 3.4 times higher in A. dracunculus "hairy" root cultures than in the control roots. The accumulation of mannitol was a special feature of the leaves of A. vulgaris and A. dracunculus control roots. A. vulgaris "hairy" root lines differed also in ART accumulation level. The increase of ART content up to 1.02 mg/g DW in comparison with the nontransformed roots (up to 0.687 mg/g DW) was observed. Thus, Agrobacterium rhizogenes-mediated genetic transformation can be used for obtaining of A. vulgaris and A. dracunculus "hairy" root culture produced ART and sugars in a higher amount than mother plants.
我们研究了遗传转化对普通蒿和龙蒿“毛状”根培养物中生物活性化合物（青蒿素及其共产物（ART）以及糖类）积累的影响。葡萄糖、果糖、蔗糖和甘露醇在普通蒿和龙蒿“毛状”根系中积累。在某些情况下，遗传转化导致了糖含量的增加或出现了对照植物中不存在的碳水化合物。普通蒿“毛状”根系中的蔗糖含量高出1.6倍。发现龙蒿“毛状”根培养物中的果糖含量比对照根高出3.4倍。甘露醇的积累是普通蒿和龙蒿对照根叶片的一个特殊特征。普通蒿“毛状”根系在ART积累水平上也存在差异。与未转化根（高达0.687mg/g干重）相比，观察到ART含量增加至1.02mg/g干重。因此，发根农杆菌介导的遗传转化可用于获得比母株产生更多ART和糖类的普通蒿和龙蒿“毛状”根培养物。
