Weihe E, Hartschuh W, Weber E
Neurosci Lett. 1985 Aug 5;58(3):347-52. doi: 10.1016/0304-3940(85)90079-5.
By the use of light microscopic immunohistochemistry it was shown that a significant population of small primary sensory afferents of guinea pig contains immunoreactivities to antisera directed against prodynorphin-opioid peptides, whereas immunoreactivities to antisera directed against opioid peptides exclusively contained in proenkephalin were absent. Immunoreactivities to antisera against different prodynorphin-opioid peptides were seen in small ganglionic cells and in small diameter fibers of spinal and trigeminal ganglia, of dorsal roots, of somatic peripheral nerve trunks and in cutaneous sensory nerves. There was evidence for colocalization of different prodynorphin-opioid peptides. High-performance liquid chromatography and the mouse vas deferens assay revealed opioid-active material (22.7 pmol Met-enkephalin equivalents per gram wet weight) in extracts of somatic peripheral nerves. The results indicate for the first time that opioid peptides derived from prodynorphin are peripheral and central neurotransmitter candidates of small primary somatosensory afferents.
运用光学显微镜免疫组织化学方法显示，豚鼠大量的初级感觉小传入纤维对针对前强啡肽阿片肽的抗血清呈现免疫反应性，而对仅含于脑啡肽原中的阿片肽的抗血清则无免疫反应性。在小神经节细胞以及脊髓和三叉神经节的小直径纤维、背根、躯体周围神经干和皮肤感觉神经中，可见针对不同前强啡肽阿片肽的抗血清的免疫反应性。有证据表明不同的前强啡肽阿片肽存在共定位。高效液相色谱法和小鼠输精管试验显示，躯体周围神经提取物中存在阿片活性物质（每克湿重22.7皮摩尔甲硫氨酸脑啡肽当量）。这些结果首次表明，源自前强啡肽的阿片肽是初级躯体感觉小传入纤维的外周和中枢神经递质候选物。
