Baraquin Alice, Zait Houria, Grenouillet Florence-Elisabeth, Moreau Elise, Hamrioui Boussad, Grenouillet Frédéric
Chrono-environnement, UMR UBFC/CNRS 6249 aff. INRA, University of Bourgogne/Franche-Comté, Besançon, France.
Parasitology & Mycology Department, Mustapha University Hospital, Algiers, Algeria.
Diagn Microbiol Infect Dis. 2017 Sep;89(1):20-25. doi: 10.1016/j.diagmicrobio.2017.06.002. Epub 2017 Jun 10.
Cystic echinococcosis (CE) is a neglected zoonotic disease, diagnosed through clinical findings, imaging techniques, and serology, for which many serological tests are available. Here we report a rapid unit assay, the immunochromatographic VIRapid® HYDATIDOSIS test (Vircell, Granada, Spain), potentially suitable for laboratories in low-prevalence or poorly equipped areas. This test was evaluated with a large retrospective cohort (224 sera), including patients suffering from CE or from other parasitic or liver diseases. The test was also assessed in routine conditions with a prospective cohort (115 sera) in areas where both cystic and alveolar echinococcoses have been diagnosed. Its performance (in terms of sensitivity, specificity, and both positive and negative likelihood ratios) was similar to an ELISA based on a crude antigen. Our study shows that this test performs adequately in the diagnostic process, when used with caution, especially regarding cross-reactivity with other parasitic diseases.
囊性棘球蚴病（CE）是一种被忽视的人畜共患病，可通过临床症状、影像学技术和血清学进行诊断，目前有多种血清学检测方法。在此，我们报告一种快速单项检测方法——免疫层析VIRapid® 包虫病检测（西班牙格拉纳达的Vircell公司生产），该方法可能适用于患病率较低或设备简陋地区的实验室。我们使用一个大型回顾性队列（224份血清样本）对该检测方法进行了评估，队列中包括患有囊性棘球蚴病或其他寄生虫病或肝病的患者。在已诊断出囊性和泡状棘球蚴病的地区，我们还使用一个前瞻性队列（115份血清样本）在常规条件下对该检测方法进行了评估。其性能（在敏感性、特异性以及阳性和阴性似然比方面）与基于粗抗原的酶联免疫吸附测定（ELISA）相似。我们的研究表明，该检测方法在诊断过程中表现良好，但使用时需谨慎，尤其是在与其他寄生虫病的交叉反应方面。
