Pan Min, Weng Yuteng, Sun Yao
Department of Implantology, School & Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, China.
Department of Implantology, School & Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, China.
Biochem Biophys Res Commun. 2017 Aug 19;490(2):356-363. doi: 10.1016/j.bbrc.2017.06.048. Epub 2017 Jun 21.
The well-known matrix protein Dentin matrix protein 1 (DMP1) is expressed by osteoblasts and osteocytes in bone, and it controls bone mineralization. Recently, it has been found that DMP1 is also expressed in other cell types, such as chondrocytes. Nestin cells are one important type of progenitor cell in bone marrow and are associated with bone remodeling. In our preliminary experiment, DMP1 could also be detected in Nestin cells in bone marrow. This study was designed to explore the effect on bone of DMP1 in Nestin cells. A transgenic mouse model with DMP1 expression driven by the Nestin promoter was generated. In vivo and in vitro experiments revealed that overexpression of DMP1 in Nestin cells could limit the proliferation and osteogenic differentiation of BMMSCs, subsequently leading to decreased bone mass. Lower expression of bone matrix protein and a lower bone deposition rate were also observed. Meanwhile, overexpression of DMP1 in Nestin cells had no influence on osteoclast activity. These data indicate that DMP1 plays negative roles in differentiation of Nestin cells and bone formation.
著名的基质蛋白牙本质基质蛋白1（DMP1）由骨中的成骨细胞和骨细胞表达，并控制骨矿化。最近，人们发现DMP1也在其他细胞类型中表达，如软骨细胞。巢蛋白细胞是骨髓中一种重要的祖细胞类型，与骨重塑有关。在我们的初步实验中，也能在骨髓中的巢蛋白细胞中检测到DMP1。本研究旨在探讨巢蛋白细胞中DMP1对骨的影响。构建了由巢蛋白启动子驱动DMP1表达的转基因小鼠模型。体内和体外实验表明，巢蛋白细胞中DMP1的过表达会限制骨髓间充质干细胞（BMMSCs）的增殖和成骨分化，随后导致骨量减少。还观察到骨基质蛋白表达降低和骨沉积率降低。同时，巢蛋白细胞中DMP1的过表达对破骨细胞活性没有影响。这些数据表明DMP1在巢蛋白细胞分化和骨形成中起负作用。
