Soyocak Ahu, Kurt Hulyam, Ozgen Merih, Turgut Cosan Didem, Colak Ertugrul, Gunes Hasan Veysi
Medical Biology and Genetic, Faculty of Medicine, Istanbul Aydin University, Turkey, 34295 Istanbul, Turkey.
Department of Medical Biology, Faculty of Medicine, Eskisehir Osmangazi University, 26480 Eskisehir, Turkey.
Gene. 2017 Sep 5;627:207-211. doi: 10.1016/j.gene.2017.06.027. Epub 2017 Jun 21.
Osteoarthritis (OA) is the most common joint disease characterized by joint pain and a progressive loss of articular cartilage. OA known as a non-inflammatory disease. Despite this the recent studies are shown synovitis and low inflammation to have a role in OA pathophysiology. The aim of this study to determine the roles of a potential therapeutic targets miRNA-146a, miRNA-155 and JNK expression levels in OA patients. Peripheral mononuclear blood cells (PBMCs) were extracted from OA patients and healthy subjects. The expression levels of miRNA-146a, miRNA-155 and JNK were quantified using by real-time PCR assay. According to study results a statistically significant increase was observed only in miRNA-155 expression level (p=0,039). However, miRNA-146a and miRNA-155 expressions increased in the progressive stages (grade 3 and grade 4) in OA patients. Our data suggests that correlation of miRNAs regulating and signal pathways can play an important role in OA pathogenesis and disease progression.
骨关节炎（OA）是最常见的关节疾病，其特征为关节疼痛和关节软骨的进行性丧失。OA被认为是一种非炎症性疾病。尽管如此，最近的研究表明滑膜炎和低度炎症在OA的病理生理学中起作用。本研究的目的是确定潜在治疗靶点miRNA-146a、miRNA-155和JNK表达水平在OA患者中的作用。从OA患者和健康受试者中提取外周血单核细胞（PBMC）。使用实时PCR测定法定量miRNA-146a、miRNA-155和JNK的表达水平。根据研究结果，仅在miRNA-155表达水平上观察到统计学上的显著增加（p = 0.039）。然而，OA患者在疾病进展阶段（3级和4级）miRNA-146a和miRNA-155的表达增加。我们的数据表明，调节miRNA的相关性和信号通路可能在OA发病机制和疾病进展中起重要作用。
