Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD, Australia.
Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD, Australia.
Nanomedicine. 2018 Oct;14(7):2355-2364. doi: 10.1016/j.nano.2017.06.006. Epub 2017 Jun 22.
Sheet-like layered double hydroxide nanoparticles (LDH NPs) have showed great potentials in biomedical applications such as nanocarriers for drug and gene delivery, biosensors and imaging agents. However, target delivery of drugs and genes using LDH NPs to the desired tumor sites is a major challenge in cancer therapy. The aim of this study is to develop a functional LDH-based nanocomposite for target delivery of siRNA to cancer cells. Mannose as a targeting moiety was firstly conjugated onto SiO-coated LDH nanocomposite. Cellular uptake data have demonstrated that siRNA is more efficiently delivered to osteosarcoma (U2OS) cells by mannose-conjugated SiO coated LDH nanocomposite (Man-SiO@LDH) compared to unmodified LDH NP. A commercial cell death-siRNA (CD-siRNA) delivered by Man-SiO@LDH can kill cancer cells more effectively. These results reveal that the Man-SiO@LDH nanocomposite is capable of target-delivering siRNA or drugs to tumor cells for more effective cancer treatment, which provides great potentials in cancer therapy.
片状层状双氢氧化物纳米粒子（LDH NPs）在生物医药领域有很大的应用潜力，如药物和基因传递的纳米载体、生物传感器和成像剂。然而，利用 LDH NPs 将药物和基因靶向递送至所需的肿瘤部位是癌症治疗中的一个主要挑战。本研究旨在开发一种基于 LDH 的功能纳米复合材料，用于将 siRNA 靶向递送至癌细胞。首先将甘露糖作为靶向部分接枝到 SiO2 包覆的 LDH 纳米复合材料上。细胞摄取数据表明，与未修饰的 LDH NP 相比，甘露糖接枝的 SiO2 包覆的 LDH 纳米复合材料（Man-SiO@LDH）能更有效地将 siRNA 递送至骨肉瘤（U2OS）细胞。由 Man-SiO@LDH 传递的商业细胞死亡-siRNA（CD-siRNA）能更有效地杀死癌细胞。这些结果表明，Man-SiO@LDH 纳米复合材料能够将 siRNA 或药物靶向递送至肿瘤细胞，从而更有效地治疗癌症，这为癌症治疗提供了巨大的潜力。
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