Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD, 4072, Australia.
School of Medicine, The University of Queensland, Brisbane, QLD, 4072, Australia.
Chemistry. 2017 Oct 12;23(57):14299-14306. doi: 10.1002/chem.201702835. Epub 2017 Sep 14.
Multifunctional nanoparticles for cancer theranosis have been widely explored for effective cancer detection and therapy. In this work, dually functionalised manganese-based layered double hydroxide nanoparticles (Mn-LDH) were examined as an effective anticancer drug/gene delivery system and for T -weighted magnetic resonance imaging (MRI) in brain cancer theranostics. Such Mn-LDH have been shown to accommodate dsDNA/siRNAs and efficiently deliver them to Neuro-2a cells (N2a). Mn-LDH have also shown high biocompatibility with low cytotoxicity. Importantly, the cell-death siRNA (CD-siRNA) delivered with Mn-LDH more efficiently kills brain cancer cells than the free CD-siRNA. Moreover, Mn-LDH can act as excellent contrast agents for MRI, with an r value of 4.47 mm s , which is even higher than that of commercial contrast agents based on Gd complexes (r =3.4 mm s ). Altogether, the high delivery efficacy and MRI contrast capability make dual-functional Mn-LDH potential bimodal agents for simultaneous cancer diagnosis and therapy.
多功能纳米粒子在癌症治疗学中得到了广泛的探索，以实现有效的癌症检测和治疗。在这项工作中，双功能化的锰基层状双氢氧化物纳米粒子（Mn-LDH）被用作有效的抗癌药物/基因传递系统，并用于脑癌治疗学中的 T1 加权磁共振成像（MRI）。研究表明，这种 Mn-LDH 可以容纳 dsDNA/siRNA，并有效地将它们递送到Neuro-2a 细胞（N2a）中。Mn-LDH 还表现出高生物相容性和低细胞毒性。重要的是，与游离 CD-siRNA 相比，用 Mn-LDH 传递的细胞死亡 siRNA（CD-siRNA）更有效地杀死脑癌细胞。此外，Mn-LDH 可以作为 MRI 的优异对比剂，r 值为 4.47 mm/s，甚至高于基于 Gd 配合物的商业对比剂（r =3.4 mm/s）。总之，高的递送效率和 MRI 对比能力使双功能化的 Mn-LDH 成为同时进行癌症诊断和治疗的潜在双模试剂。
