Department of Oral Pathology, São Leopoldo Mandic Institute and Research Center, Rua José Rocha Junqueira 13, Swift, 13045-755 Campinas, SP, Brazil.
Department of Oral Pathology, São Leopoldo Mandic Institute and Research Center, Rua José Rocha Junqueira 13, Swift, 13045-755 Campinas, SP, Brazil.
Arch Oral Biol. 2017 Oct;82:188-193. doi: 10.1016/j.archoralbio.2017.06.015. Epub 2017 Jun 15.
The aim of the present study was to establish a primary cell culture derived from polymorphous low grade adenocarcinoma (PLGA).
The neoplastic cells were derived from a 57-year-old female patient diagnosed with PLGA. A fragment of the tumor was collected and submitted to enzymatic digestion followed by centrifugation on a Percoll gradient. The cell population was characterized by means of immunofluorescence and detection of PRKD1 gene mutations.
Epifluorescence analysis of the primary culture revealed that the malignant epithelial cells were predominantly polygonal in shape and positive for cytokeratin 7, vimentin, and S100. The doubling time of the cell culture was 86.73h. The restriction digestion assay showed that the neoplastic cells possess PRKD1 gene mutations.
The establishment of primary cell culture derived from PLGA should be considered a useful tool for molecular analysis of this salivary gland tumor.
本研究旨在建立来源于多形性低度恶性腺癌（PLGA）的原代细胞培养。
从一位 57 岁女性患者的肿瘤组织中获取 PLGA 细胞，通过酶消化和 Percoll 梯度离心收集肿瘤组织碎片，然后进行原代细胞培养。通过免疫荧光检测和 PRKD1 基因突变检测来鉴定肿瘤细胞的特性。
原代细胞培养的荧光分析显示，恶性上皮细胞呈多边形，角蛋白 7、波形蛋白和 S100 阳性。细胞培养的倍增时间为 86.73h。限制酶消化分析显示，肿瘤细胞存在 PRKD1 基因突变。
PLGA 原代细胞培养的建立应被视为对这种唾液腺肿瘤进行分子分析的有用工具。
