下咽和中咽鳞状细胞癌及瘤周黏膜的比较性miRNA表达谱分析
Comparative miRNA Expression Profile Analysis of Squamous Cell Carcinoma and Peritumoral Mucosa from the Meso- and Hypopharynx.
作者信息
Orosz Eva, Gombos Katalin, Riedling Tamas, Afiakurue Priscilla, Kiss Istvan, Pytel Jozsef, Gerlinger Imre, Szanyi Istvan
机构信息
PTE KK Department of Otorhinolaryngology and Head and Neck Surgery, Pécs, Hungary
PTE ÁOK Department of Laboratory Medicine, Pécs, Hungary.
出版信息
Cancer Genomics Proteomics. 2017 Jul-Aug;14(4):285-292. doi: 10.21873/cgp.20039.
BACKGROUND/AIM: The role of microRNAs (miRNA) in carcinogenesis is related to their genome-regulatory function. The aim of the present study was to identify and compare miRNA expression signatures of meso- and hypopharynx squamous cell cancers in consideration of the cancer field hypothesis.
PATIENTS AND METHODS
Thirteen snap-frozen biopsy series of tumors and peritumoral tissues from the meso- and hypopharynx were analyzed regarding their miRNA expression with quantitative real-time polymerase chain reaction (qRT-PCR).
RESULTS
Significant miRNA expression changes of miR-21, -27a, -34a, -143 and -146a were found in peritumoral tissues that were dependent from the tumor location and the distance from the primary tumor site.
CONCLUSION
miRNA expression analysis was found to be appropriate for molecular segregation of tumor location and peritumoral tissue segments, and appears to be a promising marker for cancer field characterization.
背景/目的：微小RNA（miRNA）在致癌过程中的作用与其基因组调控功能有关。本研究的目的是根据癌场假说，鉴定并比较中咽和下咽鳞状细胞癌的miRNA表达特征。
患者与方法
采用定量实时聚合酶链反应（qRT-PCR）分析了13例中咽和下咽肿瘤及瘤周组织的冰冻活检样本的miRNA表达情况。
结果
在瘤周组织中发现miR-21、-27a、-34a、-143和-146a的miRNA表达有显著变化，这些变化取决于肿瘤位置以及与原发肿瘤部位的距离。
结论
发现miRNA表达分析适用于肿瘤位置和瘤周组织段的分子区分，并且似乎是癌场特征化的一个有前景的标志物。
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