Laboratory of Seafood Processing, Innovative and Application Institute, Zhejiang Ocean University, Zhoushan, 316022, China.
Laboratory of Food Chemistry and Nutrition, College of Food Science and Engineering, Ocean University of China, Qingdao, 266003, China.
Environ Sci Pollut Res Int. 2017 Aug;24(22):18630-18639. doi: 10.1007/s11356-017-9546-8. Epub 2017 Jun 25.
Cd resistance and bioaccumulation capacity were selected from parental Zygosaccharomyces rouxii (CRZ-0) while maintaining NaCl tolerance using protoplast mutagenesis technology. Ultraviolet-diethyl sulfate (UV-DES) cooperative mutagenesis, followed by preliminary screening and rescreening, was used to select the mutant strain CRZ-9. CRZ-9 grew better than CRZ-0 in YPD medium with 20 or 50 mg L of Cd. Scanning electron microscopy observations and flow cytometry tests indicated that CRZ-9 was more effective at eliminating reactive oxygen species (ROS) generated by Cd, which led to less cellular structural damage and lower lethality. Furthermore, compared with CRZ-0, CRZ-9 exhibited increased potential for application with higher Cd removal ratio, wider working pH range, and lower biomass dosage in Cd bioaccumulation. The mutant strain CRZ-9 possessed improved Cd resistance and bioaccumulation capacity and therefore is a promising strain to remove Cd from wastewater.
采用原生质体诱变技术，从亲本酿酒酵母（CRZ-0）中筛选出耐 Cd 性能和生物富集能力，同时保持 NaCl 耐受性。采用紫外-硫酸二乙酯（UV-DES）协同诱变，经过初步筛选和复筛，选择得到突变株 CRZ-9。在含 20 或 50mg/L Cd 的 YPD 培养基中，CRZ-9 的生长状况优于 CRZ-0。扫描电子显微镜观察和流式细胞术检测表明，CRZ-9 更有效地消除了 Cd 产生的活性氧（ROS），从而减少了细胞结构损伤和降低了致死率。此外，与 CRZ-0 相比，CRZ-9 在 Cd 生物富集中具有更高的 Cd 去除率、更宽的工作 pH 范围和更低的生物量剂量，表现出更高的应用潜力。突变株 CRZ-9 具有更好的耐 Cd 性能和生物富集能力，因此是一种从废水中去除 Cd 的有前途的菌株。
