Yu F, Wang R N, Chen X, Zheng S F, Wang Y Y, Chen Y
Key Laboratory of Clinical in Vitro Diagnostic Techniques of Zhejiang Province, Department of Clinical Laboratory, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
Key Laboratory of Clinical in Vitro Diagnostic Techniques of Zhejiang Province, Department of Clinical Laboratory, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2017 Jun 10;38(6):800-804. doi: 10.3760/cma.j.issn.0254-6450.2017.06.022.
To investigate the serotypes of Diarrheagenic (DEC) isolated from diarrheal patients in Zhejiang province and to explore the identification efficiency of serological screening methods. Serological agglutination tests were carried out in 696 strains of DEC (through the identification of virulence genes) which were selected from the Infectious Diarrhea Pathogen Monitoring Network Strain Bank of Zhejiang province, from July 2009 to June 2013. Results of virulence genes, serological identification and classification were compared. Among the 696 isolates of DEC, O antigen type was identified in 288 (41.4) isolates which belonging to 35 different 'O' serum types. H antigen was seen in 171 (24.6) isolates and determined as having 21 types. The agglutination rates of EAEC, ETEC, EPEC and EHEC isolates were 31.9 (130/408), 70.6 (127/180), 31.5 (29/92) and 14.3 (2/14), respectively and belonged to 30, 18, 15 kinds of 'O' sero-groups, respectively. One EHEC isolate was identified as O157∶H7. Serum groups were diverse for EAEC and EPEC, while relatively concentrated on ETEC. Different types of DEC might belong to the same sero-group or type. Among the 74 strains of DEC available for classification serologically, 41 isolates were in consistent with virulence gene identification and another 33 strains were not. The sero-group/type of DEC strains in Zhejiang were varied. Based on the serological screening method alone, DEC classification might end in getting the wrong answer, thus we would recommend the use of virulence gene for the purpose of identification.
调查浙江省腹泻患者分离出的致泻性大肠埃希菌（DEC）血清型，探讨血清学筛查方法的鉴定效率。对2009年7月至2013年6月从浙江省感染性腹泻病原体监测网络菌株库中选取的696株DEC（通过毒力基因鉴定）进行血清学凝集试验。比较毒力基因、血清学鉴定和分型结果。在696株DEC分离株中，鉴定出288株（41.4%）具有O抗原型，分属于35种不同的“O”血清型。171株（24.6%）分离株有H抗原，确定为21种类型。EAEC、ETEC、EPEC和EHEC分离株的凝集率分别为31.9%（130/408）、70.6%（127/180）、31.5%（29/92）和14.3%（2/14），分别属于30、18、15种“O”血清群。1株EHEC分离株鉴定为O157∶H7。EAEC和EPEC血清群多样，而ETEC相对集中。不同类型的DEC可能属于同一血清群或血清型。在74株可进行血清学分型的DEC中，41株与毒力基因鉴定一致，另33株不一致。浙江省DEC菌株的血清群/型多样。仅基于血清学筛查方法，DEC分型可能得出错误答案，因此建议使用毒力基因进行鉴定。
