Neco Heytor Victor Pereira da Costa, Teixeira Vanessa Gabryelle da Silva, da Trindade Ana Carolina Lemos, Magalhães Paula Machado Ribeiro, de Lorena Virgínia Maria Barros, Castellano Lúcio Roberto Cançado, de Souza Joelma Rodrigues, Vasconcelos Luydson Richardson, de Moura Patrícia Muniz Mendes Freire, de Morais Clarice Neuenschwander Lins
1 Department of Virology and Experimental Therapy (LaViTE), Aggeu Magalhães Institute (IAM), Oswaldo Cruz Foundation (Fiocruz) , Recife, Brazil .
2 Institute of Biological Sciences (ICB), University of Pernambuco , Recife, Brazil .
AIDS Res Hum Retroviruses. 2017 Nov;33(11):1134-1139. doi: 10.1089/AID.2016.0296. Epub 2017 Jul 25.
HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) is a chronic demyelinating and disabling syndrome caused by human T lymphotropic virus 1 (HTLV-1). Although the pathogenic mechanisms that lead to HAM/TSP outcome have not been elucidated, genetic and immunological factors may be involved in the myelopathy occurrence. This study aimed to compare cytokines, chemokines, and nitric oxide (NO) levels in asymptomatic and HAM/TSP HTLV-1-infected patients. The study group consisted of 21 HAM/TSP and 48 asymptomatic HTLV-1 patients. Chemokines (CCL5, CCL2, CXCL8, CXCL9, and CXCL10) and cytokines [IL-2, interferon gamma (IFN-γ), tumor necrosis factor alpha (TNF-α), IL-4, IL-6, and IL-10] were measured using cytometric bead array, whereas NO production was measured after reaction of supernatants with nitrate reduction solution. CXCL9 and CXCL10 chemokines levels were found to be higher in the HAM/TSP group. CXCL9 was also strongly correlated with CXCL10 and both CXCL9 and CXCL10 were moderately correlated with CCL2 and CCL5 levels, in both HAM/TSP and asymptomatic groups. There was no significant difference related to NO, IL-4, IL-6, and IL-10 levels between the clinical groups but TNF-α and IFN-γ levels were increased in HAM/TSP patients. Thus, factors such as CXCL9, CXCL10, TNF-α, and IFN-γ could be good prognostic biomarker candidates, and further studies may help to clarify their association with HAM/TSP immunopathogenesis.
人类嗜T淋巴细胞病毒1型（HTLV-1）相关脊髓病/热带痉挛性截瘫（HAM/TSP）是一种由人类嗜T淋巴细胞病毒1型（HTLV-1）引起的慢性脱髓鞘性致残综合征。尽管导致HAM/TSP发病的致病机制尚未阐明，但遗传和免疫因素可能与脊髓病的发生有关。本研究旨在比较无症状和HAM/TSP HTLV-1感染患者的细胞因子、趋化因子和一氧化氮（NO）水平。研究组由21例HAM/TSP患者和48例无症状HTLV-1患者组成。使用细胞计数珠阵列检测趋化因子（CCL5、CCL2、CXCL8、CXCL9和CXCL10）和细胞因子[白细胞介素-2、干扰素γ（IFN-γ）、肿瘤坏死因子α（TNF-α）、白细胞介素-4、白细胞介素-6和白细胞介素-10]，而在上清液与硝酸盐还原溶液反应后检测NO生成。发现HAM/TSP组中CXCL9和CXCL10趋化因子水平较高。在HAM/TSP组和无症状组中，CXCL9也与CXCL10密切相关，且CXCL9和CXCL10均与CCL2和CCL5水平中度相关。临床组之间在NO、白细胞介素-4、白细胞介素-6和白细胞介素-10水平方面无显著差异，但HAM/TSP患者的TNF-α和IFN-γ水平升高。因此，CXCL9、CXCL
