Karaki N, Aljawish A, Muniglia L, Bouguet-Bonnet S, Leclerc S, Paris C, Jasniewski J, Humeau-Virot C
Laboratoire d'Ingénierie des Biomolécules (LIBio), Université de Lorraine,2 avenue de la Forêt de Haye, TSA40602, F-54518 Vandœuvre-lès-Nancy, France.
Laboratoire de Cristallographie, Résonance Magnétique et Modélisations (CRM2), UMR 7036, Université de Lorraine, Vandœuvre-lès-Nancy F-54506, France.
Enzyme Microb Technol. 2017 Sep;104:1-8. doi: 10.1016/j.enzmictec.2017.05.001. Epub 2017 May 13.
Pectin is a natural biopolymer extracted mostly from citrus peel, sugar beet and apple pomace. In order to improve its functional properties and then to enlarge the field of its potential applications, functionalization reaction of citrus pectin with ferulic acid (FA)-oxidation products was performed in aqueous medium, at 30°C and pH7.5, in the presence of Myceliophthora thermophila laccase as biocatalyst. The conjugation between FA-oxidation products and pectin was confirmed using FTIR, UV-Vis and LC-MS analyses. The obtained results suggested that covalent bonds were between the pectin carboxyl groups and FA-oxidation products between the pectin carboxyl groups and FA-oxidation products. The determination of the total phenolic content showed that the modified pectin contained 5 times more phenols than the native pectin. In view of these results, this enzymatic procedure appears as a promising way to provide new pectin-based polymers that are expected to present new properties of interest.
果胶是一种天然生物聚合物，主要从柑橘皮、甜菜和苹果渣中提取。为了改善其功能特性，进而扩大其潜在应用领域，在嗜热毁丝霉漆酶作为生物催化剂的存在下，于30℃和pH7.5的水介质中进行了柑橘果胶与阿魏酸（FA）氧化产物的功能化反应。使用傅里叶变换红外光谱（FTIR）、紫外可见光谱（UV-Vis）和液相色谱-质谱联用（LC-MS）分析确认了FA氧化产物与果胶之间的共轭作用。所得结果表明，果胶羧基与FA氧化产物之间形成了共价键。总酚含量的测定表明，改性果胶所含的酚类物质比天然果胶多5倍。鉴于这些结果，这种酶促方法似乎是一种有前景的方式，可提供有望呈现新的有趣特性的新型果胶基聚合物。
