Barbosa Fernanda de Oliveira, Freitas Neto Oliveiro Caetano de, Batista Diego Felipe Alves, Almeida Adriana Maria de, Rubio Marcela da Silva, Alves Lucas Bocchini Rodrigues, Vasconcelos Rosemeire de Oliveira, Barrow Paul Andrew, Berchieri Junior Angelo
Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista, Jaboticabal, São Paulo, Brasil.
Departamento de Ciências Veterinárias, Universidade Federal da Paraíba, Areia, Paraiba, Brasil.
Braz J Microbiol. 2017 Oct-Dec;48(4):754-759. doi: 10.1016/j.bjm.2017.01.012. Epub 2017 Jun 7.
Salmonella Enteritidis causes fowl paratyphoid in poultry and is frequently associated to outbreaks of food-borne diseases in humans. The role of flagella and flagella-mediated motility into host-pathogen interplay is not fully understood and requires further investigation. In this study, one-day-old chickens were challenged orally with a wild-type strain Salmonella Enteritidis, a non-motile but fully flagellated (SE ΔmotB) or non-flagellated (SE ΔfliC) strain to evaluate their ability to colonise the intestine and spread systemically and also of eliciting gross and histopathological changes. SE ΔmotB and SE ΔfliC were recovered in significantly lower numbers from caecal contents in comparison with Salmonella Enteritidis at early stages of infection (3 and 5dpi). The SE ΔmotB strain, which synthesises paralysed flagella, showed poorer intestinal colonisation ability than the non-flagellated SE ΔfliC. Histopathological analyses demonstrated that the flagellated strains induced more intense lymphoid reactivity in liver, ileum and caeca. Thus, in the present study the flagellar structure and motility seemed to play a role in the early stages of the intestinal colonisation by Salmonella Enteritidis in the chicken.
肠炎沙门氏菌可引起家禽的禽副伤寒，且常与人类食源性疾病的暴发有关。鞭毛及其介导的运动在宿主与病原体相互作用中的作用尚未完全明确，需要进一步研究。在本研究中，用野生型肠炎沙门氏菌菌株、无运动能力但鞭毛完整的菌株（SE ΔmotB）或无鞭毛的菌株（SE ΔfliC）对1日龄雏鸡进行口服攻毒，以评估它们在肠道定植、全身扩散的能力，以及引发大体病变和组织病理学变化的能力。在感染早期（感染后3天和5天），与肠炎沙门氏菌相比，从盲肠内容物中回收的SE ΔmotB和SE ΔfliC数量显著减少。合成麻痹性鞭毛的SE ΔmotB菌株在肠道定植能力方面比无鞭毛的SE ΔfliC更差。组织病理学分析表明，有鞭毛的菌株在肝脏、回肠和盲肠中诱导了更强的淋巴反应。因此，在本研究中，鞭毛结构和运动似乎在肠炎沙门氏菌在雏鸡肠道定植的早期阶段发挥了作用。
