Lee Jechan, Kim Jieun, Oh Jeong-Ik, Lee Sang-Ryong, Kwon Eilhann E
Department of Environment and Energy, Sejong University, Seoul 05006, South Korea.
Advanced Technology Department, Land & Housing Institute, Daejeon 34047, South Korea.
Environ Pollut. 2017 Nov;230:81-86. doi: 10.1016/j.envpol.2017.06.042. Epub 2017 Jun 22.
This study lays great emphasis on establishing a reliable analytical platform to quantify and specify volatile fatty acids (VFAs) in the aqueous phase by derivatizing VFAs into their corresponding alkyl esters via thermally-induced rapid esterification (only 10 s reaction time). To this end, reaction conditions for the thermally-induced rapid esterification are optimized. A volumetric ratio of 0.5 at 400 °C for VFA/methanol is identified as the optimal reaction conditions to give ∼90% volatile fatty acid methyl ester (VFAME) yield. To maintain a high yield of VFAMEs, this study suggests that dilution of the sample to an optimum concentration (∼500 ppm for each VFA) is required. Derivatization of VFAs into VFAMEs via the thermally-induced rapid esterification is more reliable to quantify and specify VFAs in the aqueous phase than conventional colorimetric method.
本研究非常重视建立一个可靠的分析平台，通过热诱导快速酯化反应（反应时间仅10秒）将挥发性脂肪酸（VFA）衍生化为相应的烷基酯，从而对水相中的挥发性脂肪酸进行定量和定性分析。为此，对热诱导快速酯化反应的条件进行了优化。确定在400℃下VFA/甲醇的体积比为0.5为最佳反应条件，挥发性脂肪酸甲酯（VFAME）产率约为90%。为了保持较高的VFAME产率，本研究表明需要将样品稀释至最佳浓度（每种VFA约500 ppm）。通过热诱导快速酯化反应将VFA衍生化为VFAME，在定量和定性分析水相中的VFA方面比传统比色法更可靠。
