Crabb J W, Hanstein W G
Biochem Int. 1985 Aug;11(2):255-63.
All five subunits of bovine heart mitochondrial F1-ATPase have been isolated by reverse-phase HPLC and NH2-terminal sequences determined by gas phase Edman degradations. Bovine gamma exhibits 16 identities in the first 30 residues compared with the NH2-terminus of gamma from E.coli F1. Bovine delta exhibit about 27% identity with residues 28-59 of precursor delta from N.crassa and in the first six residues is identical with delta from S.cerevisiae. Approximately half of bovine epsilon has been sequenced. Possibly significant sequence similarities exist between bovine gamma and epsilon and kinase-related gene and oncogene products. The bovine alpha subunit has a blocked NH2-terminus.
牛心线粒体F1 - ATP酶的所有五个亚基已通过反相高效液相色谱法分离，并通过气相埃德曼降解法测定了其氨基末端序列。与大肠杆菌F1的γ亚基氨基末端相比，牛γ亚基在前30个残基中有16个相同之处。牛δ亚基与粗糙脉孢菌前体δ亚基的第28 - 59个残基有大约27%的同源性，并且在前六个残基上与酿酒酵母的δ亚基相同。牛ε亚基大约一半的序列已被测序。牛γ亚基和ε亚基与激酶相关基因及癌基因产物之间可能存在显著的序列相似性。牛α亚基的氨基末端被封闭。
