Department of Chemical Engineering, Aragon Institute of Nanoscience (INA), University of Zaragoza, Campus Río Ebro-Edificio I+D, C/Poeta Mariano Esquillor S/N, 50018-Zaragoza, Spain.
Nanoscale. 2017 Jul 20;9(28):9848-9858. doi: 10.1039/c7nr01853c.
Mesenchymal stem cells (MSCs) not only can be differentiated into different cell types but also have tropism towards injured or inflamed tissues serving as repair cells. Here we have demonstrated that MSCs containing gold nanoparticles (GNPs) whose surface has been functionalized with PEG show accelerated cell migration, successful scaffold colonization and regeneration. We report the impact of GNPs on the migration (by the wound healing assay), and proliferation (by flow cytometry analysis and by the detection of metabolic mitochondrial activity) on the behaviour of different cell lines including MSCs, HeLa cells, and human dermal fibroblasts. We conclude that GNPs are easily internalized by MSCs causing an increase in their migration rate, mediated by actin and tubulin with a 4-fold increased expression level of those proteins. We also demonstrate that MSCs containing GNPs are able to successfully colonize fibrin and PCL-based scaffolds and that an enhanced osteoblastic differentiation is reached when using the nanoparticle-laden cells compared to untreated cells used as a control. These results highlight the potential use of MSCs as therapeutic nanoparticle-carriers in regenerative medicine.
间充质干细胞（MSCs）不仅可以分化为不同的细胞类型，而且对受损或发炎的组织具有趋化性，可作为修复细胞。在这里，我们已经证明，表面经过 PEG 功能化的载金纳米粒子（GNPs）的 MSC 具有加速细胞迁移、成功支架定植和再生的能力。我们报告了 GNPs 对不同细胞系（包括 MSC、HeLa 细胞和人真皮成纤维细胞）的迁移（通过划痕愈合试验）和增殖（通过流式细胞术分析和检测代谢线粒体活性）的影响。我们得出结论，GNPs 很容易被 MSC 内化，导致其迁移率增加，这是由肌动蛋白和微管蛋白介导的，这些蛋白的表达水平增加了 4 倍。我们还证明，载有 GNPs 的 MSC 能够成功定植纤维蛋白和 PCL 支架，并且与用作对照的未处理细胞相比，使用负载纳米颗粒的细胞可达到增强的成骨细胞分化。这些结果突出了 MSC 作为治疗性纳米颗粒载体在再生医学中的潜在用途。
