Department of Oral Maxillofacial Surgery, The Affiliated Hospital of Qingdao University, Qingdao, China.
Department of Oral Maxillofacial Surgery, College of Medicine, Stomatological Hospital, Xi'an Jiaotong University, Xi'an, China.
J Oral Pathol Med. 2017 Nov;46(10):998-1003. doi: 10.1111/jop.12609. Epub 2017 Jul 24.
High mobility group AT-hook 2 (HMGA2) and pleomorphic adenoma gene 1(PLAG1) have been demonstrated to be elevated in many malignant tumors. However, the aim of this study was to evaluate HMGA2 and PLAG1 levels in blood as a non-invasive biomarker for oral squamous cell carcinoma (OSCC) diagnosis.
qRT-PCR was performed to measure circulating HMGA2 and PLAG1 levels in OSCC patients (n=43) and matched cancer-free blood control group (n=21). Clinical data of all patients were recorded.
Circulating HMGA2 and PLAG1 in the 43 OSCC patients was significantly higher than in control group (P<.001, P=.038, respectively). Furthermore, HMGA2 expression in OSCC patients with poor-moderate differentiation was increased compared with well-differentiated group. However, no significant differences in PLAG1 expression were detected when differentiation was considered. In addition, the receiver operating characteristic (ROC) curve analysis for circulating HMGA2 revealed an area under the ROC curve of 0.876 (95% confidence interval, 0.793-0.959; P<.001) with 65.1% sensitivity and 100% specificity in discriminating OSCC from controls at a cutoff value of 14.380, demonstrating significant diagnostic value for OSCC.
Circulating HMGA2 levels are increased in OSCC patients and may potentially serve as a significant index to evaluate OSCC diagnosis.
高迁移率族 AT 盒 2（HMGA2）和多形性腺瘤基因 1（PLAG1）已被证明在许多恶性肿瘤中升高。然而，本研究旨在评估血液中 HMGA2 和 PLAG1 水平作为口腔鳞状细胞癌（OSCC）诊断的非侵入性生物标志物。
qRT-PCR 用于测量 43 例 OSCC 患者和 21 例匹配的无癌血液对照组的循环 HMGA2 和 PLAG1 水平。记录所有患者的临床数据。
43 例 OSCC 患者的循环 HMGA2 和 PLAG1 明显高于对照组（P<.001，P=.038）。此外，与分化良好组相比，分化不良-中度分化的 OSCC 患者 HMGA2 表达增加。然而，当考虑分化时，PLAG1 表达没有差异。此外，循环 HMGA2 的受试者工作特征（ROC）曲线分析显示，ROC 曲线下面积为 0.876（95%置信区间，0.793-0.959；P<.001），在截断值为 14.380 时，区分 OSCC 与对照组的敏感性为 65.1%，特异性为 100%，具有显著的 OSCC 诊断价值。
OSCC 患者的循环 HMGA2 水平升高，可能作为评估 OSCC 诊断的重要指标。
