Karbasizade Vajihe, Dehghan Parichehr, Sichani Maryam Mohammadi, Shahanipoor Kahin, Jafari Reyhaneh, Yousefian Rozita
Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Microbiology, Biology of Faculty, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.
Pak J Pharm Sci. 2017 Mar;30(2(Suppl.)):585-589.
Following the increasing antibiotic resistance of pathogenic bacteria, the use of medicinal herbs as antibacterial agents has attracted growing attention. Pseudomonas aeruginosa is a human opportunistic pathogen that uses quorum sensing for regulating virulence gene expression (pyocyanin, protease, and elastase production and biofilm formation). This study examined the anti-quorum sensing activity of Quercus infectoria, Zataria multiflora and Trachyspermum copticum extracts on standard P. aeruginosa strain. The minimum inhibitory concentration (MIC) of Q. infectoria, Z. multiflora and T. copticum extracts for standard P. aeruginosa strain was determined through micro dilution. Microtiter plates were used to evaluate the anti-quorum sensing effects of the three extracts (at a sub-MIC concentration) on pyocyanin, protease, and elastase production and biofilm formation. The acetone extract of Q. infectoria showed the highest anti-quorum sensing activity and reduced the pyocyanin, protease, and elastase production and biofilm formation by 89.1%, 78%, 73.3%, and 70.1%, respectively. The corresponding values were 88.2%, 72.1%, 69%, and 61.1% for the methanol extract of Z. multiflora and 70.6%, 63.42%, 60.1%, and 59.1% for the methanol extract of T. copticum. Considering the high anti-quorum sensing activity of the studied extracts, especially the acetone extract of Q. infectoria, these herbs can be used as antipathogenic drugs.
随着病原菌对抗生素的耐药性不断增强，将药草用作抗菌剂受到了越来越多的关注。铜绿假单胞菌是一种人类机会致病菌，它利用群体感应来调节毒力基因的表达（如绿脓菌素、蛋白酶和弹性蛋白酶的产生以及生物膜的形成）。本研究检测了没食子、伊朗藏红花和藏茴香提取物对标准铜绿假单胞菌菌株的群体感应抑制活性。通过微量稀释法测定了没食子、伊朗藏红花和藏茴香提取物对标准铜绿假单胞菌菌株的最低抑菌浓度（MIC）。使用微量滴定板评估了这三种提取物（在亚MIC浓度下）对绿脓菌素、蛋白酶和弹性蛋白酶产生以及生物膜形成的群体感应抑制作用。没食子的丙酮提取物表现出最高的群体感应抑制活性，分别使绿脓菌素、蛋白酶、弹性蛋白酶的产生以及生物膜的形成减少了89.1%、78%、73.3%和70.1%。伊朗藏红花甲醇提取物的相应数值分别为88.2%、72.1%、69%和61.1%，藏茴香甲醇提取物的相应数值分别为70.6%、63.42%、60.1%和59.1%。鉴于所研究提取物，尤其是没食子的丙酮提取物具有较高的群体感应抑制活性，这些药草可作为抗病原体药物使用。
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