啮齿动物阴离子芳基硫酸酯酶的比较研究。

Comparative studies of rodent anionic arylsulfatases.

作者信息

Thompson D B, Daniel W L, Glaser J H

出版信息

Comp Biochem Physiol B. 1985;82(1):55-61. doi: 10.1016/0305-0491(85)90127-0.

Abstract

Approximately 25 and 40%, respectively, of murine (Mus musculus) and rat (Rattus norvegicus) hepatic arylsulfatase (EC 3.1.6.1) activity eluted from DEAE-ion exchange resins under high salt conditions. This high salt fraction contained arylsulfatase A and an enzyme which was immunologically similar to arylsulfatase B. The latter enzyme was thermostable, resistant to inhibition by silver, completely inhibited by phosphate, displayed linear kinetics, and had a higher pH optimum than arylsulfatase A. Anionic arylsulfatase B also hydrolyzed chondroitin-4-SO4 heptasaccharide. Sephacryl S-300 gel filtration resolved anionic arylsulfatase B into 55 and 115 kd fractions. Rodent arylsulfatase A activity was grossly underestimated when 4-methyl-umbelliferyl sulfate was employed as substrate.

摘要

在高盐条件下，分别约有25%和40%的小鼠（小家鼠）和大鼠（褐家鼠）肝脏芳基硫酸酯酶（EC 3.1.6.1）活性从DEAE离子交换树脂上洗脱下来。这个高盐组分包含芳基硫酸酯酶A和一种在免疫学上与芳基硫酸酯酶B相似的酶。后一种酶耐热，对银抑制有抗性，被磷酸盐完全抑制，呈现线性动力学，并且比芳基硫酸酯酶A有更高的最适pH值。阴离子型芳基硫酸酯酶B也能水解硫酸软骨素-4-硫酸七糖。Sephacryl S-300凝胶过滤将阴离子型芳基硫酸酯酶B分离成55和115 kd的组分。当使用4-甲基伞形酮基硫酸酯作为底物时，啮齿动物芳基硫酸酯酶A的活性被严重低估。