哺乳动物芳基硫酸酯酶A和B的比较生物化学

Comparative biochemistry of mammalian arylsulfatases A and B.

Thompson D B, Daniel W L

Department of Zoology, Arizona State University, Tempe 85287.

Comp Biochem Physiol B. 1988;90(4):823-31. doi: 10.1016/0305-0491(88)90340-9.

Abstract

Arylsulfatases A and B occurred as a major anionic and cationic isozyme, respectively, among eleven eutherian mammalian species. 2. Minor anionic arylsulfatase B isozymes were observed in rodents, dog, whale and pig, and were either monomeric (vole, Mr = 67 +/- 2 kDa), an apparent aggregate (dog, whale, pig; Mr = 192 +/- 10 kDa), or both (rat, mouse; monomeric Mr = 57 +/- 2 kDa; apparent dimeric Mr = 114 +/- 3 kDa). 3. Minor cationic arylsulfatase A isozymes were isolated from the deer, whale and pig. 4. Opossum arylsulfatases A and B were both anionic, had similar relative molecular weights, were not inhibited by silver, and were not precipitated by anti-murine arylsulfatase B nor anti-bovine arylsulfatase A IgG preparations.

摘要

在11种真兽亚纲哺乳动物中，芳基硫酸酯酶A和B分别作为主要的阴离子和阳离子同工酶出现。2. 在啮齿动物、狗、鲸和猪中观察到少量阴离子型芳基硫酸酯酶B同工酶，它们要么是单体（田鼠，Mr = 67 +/- 2 kDa），明显是聚集体（狗、鲸、猪；Mr = 192 +/- 10 kDa），要么两者都有（大鼠、小鼠；单体Mr = 57 +/- 2 kDa；明显的二聚体Mr = 114 +/- 3 kDa）。3. 从鹿、鲸和猪中分离出少量阳离子型芳基硫酸酯酶A同工酶。4. 负鼠的芳基硫酸酯酶A和B都是阴离子型的，相对分子量相似，不受银抑制，也不被抗鼠芳基硫酸酯酶B或抗牛芳基硫酸酯酶A IgG制剂沉淀。