Hassoni A A, Kerkut G A, Walker R J
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1985;82(1):179-92. doi: 10.1016/0742-8413(85)90227-0.
Strips of muscle, approximately 12 segments in length, were prepared from the body wall of the earthworm, Lumbricus terrestris, from which the nerve cord and viscera had been removed. Contractions to electrical stimulation and acetylcholine agonists were recorded using an isometric transducer. A range of nicotinic and muscarinic agonists and antagonists were tested on this preparation and the results indicate that the acetylcholine receptor on this muscle cannot be classified as either nicotinic or muscarinic. Hemicholinium-3 abolished electrically induced muscle twitches at concentrations which had no effect on the acetylcholine response. Alpha-Bungarotoxin blocked the responses to both electrical stimulation and acetylcholine while beta-bungarotoxin blocked the contractions induced by electrical stimulation but potentiated the acetylcholine contraction.
从去除了神经索和内脏的蚯蚓（陆正蚓）体壁制备出长度约为12节的肌肉条。使用等长换能器记录对电刺激和乙酰胆碱激动剂的收缩反应。在此制备物上测试了一系列烟碱样和毒蕈碱样激动剂及拮抗剂，结果表明该肌肉上的乙酰胆碱受体既不能归类为烟碱样受体也不能归类为毒蕈碱样受体。半胱胺酸-3在对乙酰胆碱反应无影响的浓度下消除了电诱导的肌肉抽搐。α-银环蛇毒素阻断了对电刺激和乙酰胆碱的反应，而β-银环蛇毒素阻断了电刺激诱导的收缩，但增强了乙酰胆碱诱导的收缩。
