Gingle A R
Comp Biochem Physiol C Comp Pharmacol Toxicol. 1985;82(1):235-41. doi: 10.1016/0742-8413(85)90237-3.
Growth yield in cultures of Schneider's line 2 cells was studied as a function of plating cell density. It was measured in the presence of various neurotransmitters, hormones and related chemicals. Of these, acetylcholine and carnitine were studied in detail. Both significantly increased growth yields at low plating cell densities. Atropine and tubocurarine significantly reduced growth yield, suggesting the presence of a cholinergic-like receptor. Cell populations were incubated with [14C]choline and uptake occurred. The [14C]choline was incorporated into the production of secreted molecules which comigrated with carnitine offering evidence for carnitine production and secretion.
研究了施奈德2号线细胞培养物中的生长产量与接种细胞密度的关系。在存在各种神经递质、激素和相关化学物质的情况下对其进行了测量。其中，对乙酰胆碱和肉碱进行了详细研究。在低接种细胞密度下，二者均显著提高了生长产量。阿托品和筒箭毒碱显著降低了生长产量，这表明存在一种胆碱能样受体。细胞群体用[14C]胆碱进行孵育并发生摄取。[14C]胆碱被整合到与肉碱迁移率相同的分泌分子的产生过程中，这为肉碱的产生和分泌提供了证据。
