Shu Chin-Chung, Wu Ming-Fang, Wang Jann-Yuan, Lai Hsin-Chih, Lee Li-Na, Chiang Bor-Luen, Yu Chong-Jen
Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, Taiwan.
Department of Traumatology, National Taiwan University Hospital, Taipei, Taiwan.
Respir Res. 2017 Jun 26;18(1):128. doi: 10.1186/s12931-017-0580-3.
Tuberculosis (TB) is one of the most common infectious diseases worldwide. During active tuberculosis, T helper (Th) 17 cells are decreased, however the association with inhibitory immune regulation is unclear.
We enrolled 27 patients with TB and 20 age- and sex-matched controls and studies their lymphocyte status. Peripheral blood lymphocytes were isolated and programmed death-1 (PD-1) and programmed death ligand 1 (PD-L1) were measured on Th17 cells by using flow cytometry after the cells were stimulated with phorbol 12-myristate 13-acetate and ionomycin for 6 h. In addition, Th2 and regulatory T cells were measured and analyzed.
The TB group had lower levels of Th17 cells but higher levels of Th2 and Treg cells than the controls. In Th17 cells, the percentage of PD-L1 was higher in the TB group than that in the controls. In Th2 and Treg cells, the percentage of cytotoxic T-lymphocyte associated protein 4 (CTLA-4) was lower in the TB group and PD-1 was higher in Treg cells in the TB group. In the patients with extra-pulmonary TB, levels of Th1, Th2 and T17 cells were lower than those with pulmonary TB. The percentage of PD-1 on Th1 lymphocytes positively correlated with radiographic score.
Lower level of Th17 in TB patients may be associated with increased percentage of PD-L1 and increasing levels of Th2 and Treg cells which influenced by CTLA-4.
结核病是全球最常见的传染病之一。在活动性结核病期间，辅助性T（Th）17细胞减少，但其与免疫抑制调节的关系尚不清楚。
我们招募了27例结核病患者和20例年龄及性别匹配的对照者，并研究了他们的淋巴细胞状态。分离外周血淋巴细胞，在用佛波酯12-肉豆蔻酸酯13-乙酸酯和离子霉素刺激细胞6小时后，使用流式细胞术检测Th17细胞上的程序性死亡受体1（PD-1）和程序性死亡配体1（PD-L1）。此外，还对Th2细胞和调节性T细胞进行了检测和分析。
与对照组相比，结核病组的Th17细胞水平较低，但Th2细胞和调节性T细胞水平较高。在Th17细胞中，结核病组的PD-L1百分比高于对照组。在Th2细胞和调节性T细胞中，结核病组的细胞毒性T淋巴细胞相关蛋白4（CTLA-4）百分比较低，而调节性T细胞中的PD-1较高。在肺外结核病患者中，Th1、Th2和Th17细胞水平低于肺结核患者。Th1淋巴细胞上PD-1的百分比与影像学评分呈正相关。
结核病患者Th17水平较低可能与PD-L1百分比增加以及受CTLA-4影响的Th2细胞和调节性T细胞水平升高有关。
