Chiari E, de Souza W, Romanha A J, Chiari C A, Brener Z
Acta Trop. 1978 Jun;35(2):113-21.
Epimastigotes and trypomastigotes of T. cruzi obtained from acellular culture as well as bloodstream trypomastigotes agglutinate with concanavalin A (Con A). Con A-binding sites were also localized on the cell membrane by using the Con A-horseradish peroxidase-diaminobenzidine method. Passage of epimastigotes and trypomastigotes from acellular culture through DEAE-cellulose column did not affect Con A-binding sites as detected by agglutination and electron miscroscopy.
从无细胞培养物中获得的克氏锥虫的前鞭毛体和锥鞭毛体以及血流中的锥鞭毛体可与刀豆球蛋白A(Con A)发生凝集反应。通过Con A-辣根过氧化物酶-二氨基联苯胺法,还可将Con A结合位点定位在细胞膜上。通过凝集反应和电子显微镜检测发现,无细胞培养物中的前鞭毛体和锥鞭毛体通过DEAE-纤维素柱传代后,Con A结合位点并未受到影响。