Choubey Praween Kumar, Roy Jagat Kumar
Cytogenetics Laboratory, Department of Zoology, Banaras Hindu University, Varanasi, 221 005, Uttar Pradesh, India.
Genesis. 2017 Aug;55(8). doi: 10.1002/dvg.23045. Epub 2017 Jul 14.
Intracellular vesicular trafficking is one of the important tools in maintaining polarity, adhesion, and shape of epithelial cells. Rab11, a subfamily of the Ypt/Rab gene family of ubiquitously expressed GTPases and a molecular marker of recycling endosomes, transports different components of plasma membrane. Here, we report that Rab11 affects tubulogenesis of Malpighian tubules (MTs). MTs are simple polarized epithelial tubular structures, considered as functional analogue of human kidney. Rab11 has pleiotropic effects on MTs development as down-regulation of Rab11 in principal cells (PCs) of MTs from embryonic stages of development results in reduced endoreplication, clustering of cells, disorganized cytoskeleton, and disruption of polarity leading to shortening of MTs in third instar larvae. Rab11 is also required for proper localization of different transporters in PCs, essential for physiological activity of MTs. Collectively, our data suggest that Rab11 plays a key role in the process of tubulogenesis of MTs in Drosophila.
细胞内囊泡运输是维持上皮细胞极性、黏附性和形状的重要机制之一。Rab11是Ypt/Rab基因家族的一个亚家族，该家族是普遍表达的GTP酶，也是再循环内体的分子标记，负责运输质膜的不同成分。在此，我们报告Rab11影响马氏管（MT）的微管形成。MT是简单的极化上皮管状结构，被认为是人类肾脏的功能类似物。Rab11对MT的发育具有多效性影响，因为在MT主要细胞（PC）中从胚胎发育阶段下调Rab11会导致内复制减少、细胞聚集、细胞骨架紊乱以及极性破坏，从而导致三龄幼虫的MT缩短。Rab11也是PC中不同转运蛋白正确定位所必需的，这对MT的生理活动至关重要。总体而言，我们的数据表明Rab11在果蝇MT微管形成过程中起关键作用。
