Le Thanh Binh, Beaufay Claire, Nghiem Duc Trong, Mingeot-Leclercq Marie-Paule, Quetin-Leclercq Joëlle
GNOS Research Group, Louvain Drug Research Institue, Université Catholique de Louvain, Bruxelles 1200, Belgium.
Department of Pharmacognosy, Hanoi University of Pharmacy, 13-15 Le Thanh Tong, Hoan Kiem, Hanoi 100000, Vietnam.
Molecules. 2017 Jun 27;22(7):1071. doi: 10.3390/molecules22071071.
is one of the pathogens causing cutaneous leishmaniasis which is associated with patient morbidity. In our researches for new safe and effective treatments, thirty-seven essential oils (EOs) extracted from Vietnamese plants were screened in vitro for the first time on promastigotes at the maximum concentration of 50 nL/mL. Active EOs were also analyzed for cytotoxicity on mammalian cell lines (WI38, J774) and their selectivity indices (SI) were calculated. Their composition was determined by GC-MS and GC-FID. Our results indicated that EOs extracted from , , and , possessed a moderate anti-leishmanial activity, with IC values of 2.92 ± 0.08, 3.34 ± 0.34, 8.49 ± 0.32 and 9.25 ± 0.64 nL/mL respectively. However, they also showed cytotoxicity with SI < 10. The most promising EO was extracted from , displaying an IC of 4.85 ± 1.65 nL/mL and SI > 10. It contained 86.5% eugenol, which was demonstrated to be effective on with IC of 2.57 ± 0.57 nL/mL and not toxic on mammalian cells, explaining the observed activity.
是引起皮肤利什曼病的病原体之一，与患者发病有关。在我们寻找新的安全有效治疗方法的研究中，首次对从越南植物中提取的37种精油（EOs）进行了体外筛选，以50 nL/mL的最大浓度作用于前鞭毛体。还分析了活性精油对哺乳动物细胞系（WI38、J774）的细胞毒性，并计算了它们的选择性指数（SI）。通过气相色谱-质谱联用仪（GC-MS）和气相色谱-火焰离子化检测器（GC-FID）确定了它们的成分。我们的结果表明，从[植物名称1]、[植物名称2]、[植物名称3]和[植物名称4]中提取的精油具有中等抗利什曼原虫活性，IC值分别为2.92±0.08、3.34±0.34、8.49±0.32和9.25±0.64 nL/mL。然而，它们也表现出细胞毒性，SI<10。最有前景的精油是从[植物名称5]中提取得到的，其IC值为4.85±1.65 nL/mL，SI>10。它含有86.5%的丁香酚，已证明对[相关对象]有效，IC值为2.57±0.57 nL/mL，且对哺乳动物细胞无毒，这解释了观察到的活性。
