Sang Ming, Wang Xuanbin, Zhang Hanyao, Sun Xiaodong, Ding Xudong, Wang Puqing, Jiao Rong, Cheng Huaxian, Yang Sijun, Zhang Guibin
1 Central Laboratory of Xiangyang No. 1 People's Hospital, Institute of Parkinson's Disease, Hubei Key Laboratory of Wudang Local Chinese Medicine Research, Hubei University of Medicine , Shiyan, People's Republic of China .
2 College of Forestry, Key Laboratory of Biodiversity Conservation in Southwest China, Southwest Forestry University , Kunming, People's Republic of China .
DNA Cell Biol. 2017 Aug;36(8):647-654. doi: 10.1089/dna.2017.3650. Epub 2017 Jun 27.
RNA-sequencing, a powerful tool, yields a comprehensive view of whole transcriptome. Intracerebral hemorrhage (ICH) is a devastating form of stroke. To date, RNA-sequencing analysis of ICH has not been reported. Peripheral blood mononuclear cells (PBMCs) were used as a source of mRNA for gene expression profile analysis in stroke. In this study, we performed transcriptome analyses for PBMCs from four ICH patients and four healthy volunteers on Illumina platform. We identified 4040 significantly differentially expressed genes (DEGs). Functional annotation of DEGs with DAVID Bioinformatics Resources indicated that genes associated with cell apoptosis, autophagy, cell-cell adhesion, inflammatory response, protein binding, positive regulation of gene expression, and signal transduction were most significantly enriched by DEGs. Gene set enrichment analysis identified 40 significant Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, including chemokine signaling, cytokine-cytokine receptor interaction, oxidative phosphorylation, and glutathione metabolism processes. These data point to a complex mechanism for ICH pathogenesis. Overall, the present study demonstrated an altered gene expression profile of PBMCs in response to acute ICH. Our study provided important information for understanding the molecular mechanisms of ICH pathogenesis at system-wide levels.
RNA测序是一种强大的工具，可全面呈现整个转录组的情况。脑出血（ICH）是一种毁灭性的中风形式。迄今为止，尚未有关于脑出血的RNA测序分析报道。外周血单核细胞（PBMCs）被用作中风基因表达谱分析的mRNA来源。在本研究中，我们在Illumina平台上对4例脑出血患者和4名健康志愿者的PBMCs进行了转录组分析。我们鉴定出4040个显著差异表达基因（DEGs）。利用DAVID生物信息学资源对DEGs进行功能注释表明，与细胞凋亡、自噬、细胞间粘附、炎症反应、蛋白质结合、基因表达的正调控和信号转导相关的基因被DEGs显著富集。基因集富集分析确定了40条显著的京都基因与基因组百科全书（KEGG）通路，包括趋化因子信号传导、细胞因子-细胞因子受体相互作用、氧化磷酸化和谷胱甘肽代谢过程。这些数据指向脑出血发病机制的复杂机制。总体而言，本研究证明了急性脑出血后PBMCs基因表达谱的改变。我们的研究为在全系统水平上理解脑出血发病机制的分子机制提供了重要信息。
