Kudryavtseva Valeriya L, Zhao Li, Tverdokhlebov Sergei I, Sukhorukov Gleb B
National Research Tomsk Polytechnic University, RASA Center in Tomsk, Tomsk, 634050, Russia; National Research Tomsk Polytechnic University, Department of Experimental Physics, Tomsk, 634050, Russia.
School of Engineering and Materials Science, Queen Mary University of London, Mile End Road, London, E1 4NS, United Kingdom.
Colloids Surf B Biointerfaces. 2017 Sep 1;157:481-489. doi: 10.1016/j.colsurfb.2017.06.011. Epub 2017 Jun 17.
We propose the use of polylactic acid/calcium carbonate (PLA/CaCO) hybrid micro-particles for achieving improved encapsulation of water-soluble substances. Biodegradable porous CaCO microparticles can be loaded with wide range of bioactive substance. Thus, the formation of hydrophobic polymeric shell on surface of these loaded microparticles results on encapsulation and, hence, sealing internal cargo and preventing their release in aqueous media. In this study, to encapsulate proteins, we explore the solid-in-oil-in-water emulsion method for fabricating core/shell PLA/CaCO systems. We used CaCO particles as a protective core for encapsulated bovine serum albumin, which served as a model protein system. We prepared a PLA coating using dichloromethane as an organic solvent and polyvinyl alcohol as a surfactant for emulsification; in addition, we varied experimental parameters such as surfactant concentration and polymer-to-CaCO ratio to determine their effect on particle-size distribution, encapsulation efficiency and capsule permeability. The results show that the particle size decreased and the size distribution narrowed as the surfactant concentration increased in the external aqueous phase. In addition, when the CaCO/PLA mass ratio dropped below 0.8, the hybrid micro-particles were more likely to resist treatment by ethylenediaminetetraacetic acid and thus retained their bioactive cargos within the polymer-coated micro-particles.
我们建议使用聚乳酸/碳酸钙（PLA/CaCO）杂化微粒来改善水溶性物质的包封效果。可生物降解的多孔碳酸钙微粒能够负载多种生物活性物质。因此，在这些负载微粒表面形成疏水性聚合物外壳可实现包封，从而密封内部物质并防止其在水性介质中释放。在本研究中，为了包封蛋白质，我们探索了水包油包固乳液法来制备核壳结构的PLA/CaCO体系。我们使用碳酸钙颗粒作为包封牛血清白蛋白的保护核，牛血清白蛋白作为模型蛋白质体系。我们以二氯甲烷为有机溶剂、聚乙烯醇为乳化表面活性剂制备了PLA涂层；此外，我们改变了诸如表面活性剂浓度和聚合物与碳酸钙的比例等实验参数，以确定它们对粒径分布、包封效率和胶囊渗透性的影响。结果表明，随着外部水相中表面活性剂浓度的增加，粒径减小且粒径分布变窄。此外，当碳酸钙与PLA的质量比降至0.8以下时，杂化微粒更有可能抵抗乙二胺四乙酸的处理，从而将其生物活性物质保留在聚合物包覆的微粒内。
