Institut de Chimie et des Matériaux Paris-Est, UMR 7182 CNRS-Université Paris-Est Créteil Val-de-Marne, 2 rue Henri Dunant, 94320 Thiais, France; Laboratoire Eau Environnement et Systèmes Urbains (LEESU), Université-Paris-Est Créteil, Créteil cedex 94010, France.
Laboratoire Eau Environnement et Systèmes Urbains (LEESU), Université-Paris-Est Créteil, Créteil cedex 94010, France.
Int J Pharm. 2020 Apr 15;579:119175. doi: 10.1016/j.ijpharm.2020.119175. Epub 2020 Feb 25.
Porous calcium carbonate (CaCO) particles have been shown to be highly advantageous for biological applications, mainly due to their large surface area and their stability in physiological media. Also, developing appropriate antibacterial materials presenting the benefits of non-formation of harmful compounds is of major interest. Two characteristics of CaCO particles were investigated herein: (i) antibiotic-loading capacity and (ii) the possibility of using CaCO particles as a template for the fabrication of biocapsules presenting inherent antibacterial capacity. The particles were tested against two representative pathogenic bacteria (Staphylococcus aureus and Escherichia coli). On one hand, a method for antibiotic (namely penicillin, ampicillin and ciprofloxacin) loading inside calcium carbonate particles was developed and antibacterial activity was investigated. Encapsulation efficiency and loading content were 95% and 5%, respectively. We showed that antibiotics prevented bacterial growth within 2 h, with no evidence of bacterial regrowth within 16 h; bactericidal effects were also observed. On the other hand, the self-assembly of charged polysaccharides, namely chitosan (chi) and dextran sulfate (dex), were assessed on calcium carbonate microparticles used as a sacrificial matrix. During bacterial growth in a liquid medium, an inhibitory effect of these particles was observed, i.e. Staphylococcus aureus (Gram-positive) (from 16.3% to 48.8% for (chi/dex)-chi coated CaCO materials and from 41.9% to 93.0% for (chi/dex)-chi capsules) and Escherichia coli (Gram-negative) (from 18.2% to 45.5% for (chi/dex)-chi coated CaCO materials and from 40.0% to 89.1% for (chi/dex)-chi capsules). Staining with acridine orange highlighted the bactericidal effect of the designed particles. These findings demonstrate the excellent potential of using calcium carbonate particles in antibiotic therapy as a starting point for the development of smart materials.
多孔碳酸钙 (CaCO) 颗粒因其具有较大的表面积和在生理介质中的稳定性，已被证明在生物应用中具有巨大优势。此外，开发具有不形成有害化合物的优势的合适抗菌材料也是主要关注点。本文研究了 CaCO 颗粒的两个特性：（i）抗生素负载能力和（ii）将 CaCO 颗粒用作制备具有固有抗菌能力的生物胶囊的模板的可能性。将这些颗粒分别针对两种代表性的致病性细菌（金黄色葡萄球菌和大肠杆菌）进行了测试。一方面，开发了一种将抗生素（即青霉素、氨苄西林和环丙沙星）负载到碳酸钙颗粒内部的方法，并研究了其抗菌活性。封装效率和负载含量分别为 95%和 5%。我们表明，抗生素可在 2 小时内阻止细菌生长，16 小时内没有细菌重新生长的迹象；还观察到杀菌效果。另一方面，评估了带电荷多糖，即壳聚糖（chi）和硫酸葡聚糖（dex），在用作牺牲基质的碳酸钙微球上的自组装。在液体培养基中细菌生长期间，观察到这些颗粒具有抑制作用，即金黄色葡萄球菌（阳性菌）（（chi/dex）-chi 涂覆的 CaCO 材料从 16.3%到 48.8%，（chi/dex）-chi 胶囊从 41.9%到 93.0%）和大肠杆菌（阴性菌）（（chi/dex）-chi 涂覆的 CaCO 材料从 18.2%到 45.5%，（chi/dex）-chi 胶囊从 40.0%到 89.1%）。吖啶橙染色突出了设计颗粒的杀菌效果。这些发现证明了使用碳酸钙颗粒作为抗生素治疗的起点来开发智能材料的巨大潜力。
