Liu Na, Zhou Qi, Qi Yan-Hua, Wang Hua, Yang Li, Fan Qing-Yu
Department of Ultrasound, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, PR China.
Department of Ultrasound, Xi'an Children's Hospital, Xi'an 710003, PR China.
Exp Mol Pathol. 2017 Aug;103(1):71-77. doi: 10.1016/j.yexmp.2017.06.004. Epub 2017 Jun 24.
This study aims to explore the effects of long non-coding RNA H19 (lncRNA H19) and microRNA let7a (miRNA let7a) expression on the prognosis of thyroid cancer (TC). This may aid in the discovery of more effective treatment and prognosis approaches for TC. Between January 2008 and January 2011, 131 TC tissues and adjacent tissues were obtained from TC patients. An additional 122 normal thyroid tissues were also collected as normal controls from patients with benign thyroid lesions. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect lncRNA H19 and miRNA let7a mRNA expression. Five-year follow-ups were conducted. A Receiver operating characteristic (ROC) curve was used to evaluate the diagnostic values of lncRNA H19 and miRNA let7a in TC. The Kaplan-Meier method was applied to analyze the 5-year survival rate of TC patients. Univariate and multivariate factor analyses were employed to analyze the prognostic factors of TC. The lncRNA H19 mRNA expression was higher while the miRNA let7a mRNA expression was lower in TC tissues than, in the normal thyroid tissues and adjacent tissues. The area under the ROC curve (AUC) of lncRNA H19 and miRNA let7a were 0.801 and 0.116, with sensitivity at 72.5% and 84%, as well as specificity 75.4% and 77%, respectively. In TC patients with tumor diameters≥1.0cm, lncRNA H19 mRNA expression was elevated, but miRNA let7a mRNA expression was reduced. This was also evident in TC patients with TNM stages III+IV and those with lymph node metastasis. TC patients with a lower 5-year survival rate showed upregulated levels of lncRNA H19 expression and, downregulated levels of miRNA let7a expression. LncRNA H19 and miRNA let7a expression, tumor diameter, TNM stage and lymph node metastasis were independent prognostic factors of TC. This study demonstrated that increased lncRNA H19 and decreased miRNA let7a expression levels are associated with poor prognosis in TC patients. An inverse relationship between lncRNA H19 and miRNA let7a expression levels was exhibited.
本研究旨在探讨长链非编码RNA H19（lncRNA H19）和微小RNA let7a（miRNA let7a）表达对甲状腺癌（TC）预后的影响。这可能有助于发现更有效的TC治疗和预后方法。2008年1月至2011年1月期间，从TC患者处获取了131份TC组织及癌旁组织。另外还从甲状腺良性病变患者处收集了122份正常甲状腺组织作为正常对照。采用定量实时聚合酶链反应（qRT-PCR）检测lncRNA H19和miRNA let7a mRNA表达。进行了为期5年的随访。采用受试者工作特征（ROC）曲线评估lncRNA H19和miRNA let7a在TC中的诊断价值。应用Kaplan-Meier法分析TC患者的5年生存率。采用单因素和多因素分析方法分析TC的预后因素。与正常甲状腺组织和癌旁组织相比，TC组织中lncRNA H19 mRNA表达较高，而miRNA let7a mRNA表达较低。lncRNA H19和miRNA let7a的ROC曲线下面积（AUC）分别为0.801和0.116，敏感性分别为72.5%和84%，特异性分别为75.4%和77%。在肿瘤直径≥1.0cm的TC患者中，lncRNA H19 mRNA表达升高，但miRNA let7a mRNA表达降低。在TNM分期为III + IV期的TC患者和有淋巴结转移的患者中也很明显。5年生存率较低的TC患者显示lncRNA H19表达水平上调，miRNA let7a表达水平下调。lncRNA H19和miRNA let7a表达、肿瘤直径、TNM分期和淋巴结转移是TC的独立预后因素。本研究表明，lncRNA H19表达增加和miRNA let7a表达降低与TC患者预后不良相关。lncRNA H19和miRNA let7a表达水平之间呈负相关。
