Labak I, Pavić V, Zjalić M, Blažetić S, Viljetić B, Merdić E, Heffer M
Department of Biology, J. J. Strossmayer in Osijek, Ulica cara Hadrijana 8/A, 31000, Osijek, Croatia.
Department of Medical Chemistry, Biochemistry and Clinical Chemistry, J. J. Strossmayer in Osijek, Faculty of Medicine, Ulica cara Hadrijana 10, 31000, Osijek, Croatia.
J Fish Biol. 2017 Aug;91(2):473-489. doi: 10.1111/jfb.13352. Epub 2017 Jun 27.
In this study, tangential migration and neuronal connectivity organization were analysed in the optic tectum of seven different teleosts through the expression of polysialylated neural cell adhesion molecule (PSA-NCAM) in response to ecological niche and use of vision. Reduced PSA-NCAM expression in rainbow trout Oncorhynchus mykiss optic tectum occurred in efferent layers, while in pike Esox lucius and zebrafish Danio rerio it occurred in afferent and efferent layers. Zander Sander lucioperca and European eel Anguilla anguilla had very low PSA-NCAM expression in all tectal layers except in the stratum marginale. Common carp Cyprinus carpio and wels catfish Silurus glanis had the same intensity of PSA-NCAM expression in all tectal layers. The optic tectum of all studied fishes was also a site of tangential migration with sustained PSA-NCAM and c-series ganglioside expression. Anti-c-series ganglioside immunoreactivity was observed in all tectal layers of all analysed fishes, even in layers where PSA-NCAM expression was reduced. Since the optic tectum is indispensable for visually guided prey capture, stabilization of synaptic contact and decrease of neurogenesis and tangential migration in the visual map are an expected adjustment to ecological niche. The authors hypothesize that this stabilization would probably be achieved by down-regulation of PSA-NCAM rather than c-series of ganglioside.
在本研究中，通过多唾液酸神经细胞黏附分子（PSA-NCAM）的表达，分析了七种不同硬骨鱼视顶盖中的切向迁移和神经元连接组织，以探究其对生态位和视觉利用的响应。虹鳟鱼（Oncorhynchus mykiss）视顶盖中PSA-NCAM表达的降低出现在传出层，而在梭鲈（Esox lucius）和斑马鱼（Danio rerio）中，其表达降低出现在传入层和传出层。桑氏魮脂鲤（Sander lucioperca）和欧洲鳗鲡（Anguilla anguilla）除边缘层外，所有顶盖层中的PSA-NCAM表达都非常低。鲤鱼（Cyprinus carpio）和六须鲶（Silurus glanis）在所有顶盖层中的PSA-NCAM表达强度相同。所有研究鱼类的视顶盖也是切向迁移的部位，持续表达PSA-NCAM和c系列神经节苷脂。在所有分析鱼类的所有顶盖层中均观察到抗c系列神经节苷脂免疫反应，即使在PSA-NCAM表达降低的层中也是如此。由于视顶盖对于视觉引导的猎物捕获不可或缺，因此视觉图谱中突触接触的稳定以及神经发生和切向迁移的减少是对生态位的一种预期调整。作者推测，这种稳定可能是通过下调PSA-NCAM而非c系列神经节苷脂来实现的。
