人及兔胃黏膜细胞单层培养中胃蛋白酶原的合成
Pepsinogen synthesis in monolayer culture of human and rabbit gastric mucosal cells.
作者信息
Defize J, Arwert F, Kortbeek H, Frants R R, Meuwissen S G, Eriksson A W
出版信息
Virchows Arch B Cell Pathol Incl Mol Pathol. 1985;49(3):225-30. doi: 10.1007/BF02912099.
PMID:2865845
Abstract
We have established monolayer cultures of human and rabbit gastric mucosal cells and of isolated rabbit gastric chief cells. These cultures were capable of de novo pepsinogen synthesis and secretion, demonstrated by electrophoresis and subsequent autoradiography of cell lysates and growth medium after culture in the presence of 14C-labelled amino acids. Cultures could be maintained for 1 week without overgrowth by fibroblasts.
摘要
我们已经建立了人及兔胃黏膜细胞以及分离的兔胃主细胞的单层培养物。这些培养物能够从头合成和分泌胃蛋白酶原，这在含有¹⁴C标记氨基酸的培养基中培养后，通过对细胞裂解物和生长培养基进行电泳及随后的放射自显影得以证明。培养物可以维持1周而不会被成纤维细胞过度生长。
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