Ramaraj Vijayakumar, Vijayaraman Rajyoganandh S, Elavarashi Elangovan, Rangarajan Sudha, Kindo Anupma Jyoti
Scholar, Department of Microbiology, Sri Ramachandra Medical College and Research Institute, SRU, Chennai, Tamil Nadu, India.
Lecturer, Department of Biotechnology, Faculty of Biomedical Sciences, Technology and Research, SRU, Chennai, Tamil Nadu, India.
J Clin Diagn Res. 2017 May;11(5):DC04-DC09. doi: 10.7860/JCDR/2017/21994.9843. Epub 2017 May 1.
Dermatophytes are a group of fungi which infect keratinized tissues and causes superficial mycoses in humans and animals. The group comprises of three major genera, , and . Among them is a predominant anthropophilic fungi which causes chronic infections. Although, the infection is superficial and treatable, reinfection/coinfection causes inflation in the treatment cost. Identifying the source and mode of transmission is essential to prevent its transmission. Accurate discrimination is required to understand the clinical (relapse or reinfection) and epidemiological implications of the genetic heterogeneity of this species. Polymorphism in the Non Transcribed Spacer (NTS) region of ribosomal DNA (rDNA) clusters renders an effective way to discriminate strains among .
To carry out the strain typing of the clinical isolates, using NTS as a molecular marker.
Seventy clinical isolates obtained from April-2011-March 2013, from Sri Ramachandra Medical Centre, Chennai, Tamil Nadu, India, were identified by conventional phenotypic methods and included in this prospective study. The isolates were then subjected to Polymerase Chain Reaction (PCR) targeting two subrepeat elements (SREs), TRS-1 and TRS-2 of the NTS region.
Strain-specific polymorphism was observed in both subrepeat loci. Total, nine different strains were obtained on combining both TRS-1 and TRS-2, SREs.
The outcome has given a strong representation for using NTS region amplification in discriminating the clinical isolates. The method can be adapted as a tool for conducting epidemiology and population based study in infections. This will help in future exploration of the epidemiology of .
皮肤癣菌是一类感染角质化组织并在人和动物中引起浅表真菌病的真菌。该类包括三个主要属，即毛癣菌属、小孢子菌属和表皮癣菌属。其中毛癣菌属是主要的嗜人真菌，可引起慢性感染。尽管这种感染是浅表性的且可治疗，但再感染/合并感染会导致治疗成本增加。识别传染源和传播方式对于预防其传播至关重要。需要进行准确鉴别以了解该物种遗传异质性的临床（复发或再感染）和流行病学意义。核糖体DNA（rDNA）簇的非转录间隔区（NTS）区域的多态性为区分毛癣菌属菌株提供了一种有效方法。
以NTS作为分子标记对临床分离株进行菌株分型。
从印度泰米尔纳德邦金奈的斯里兰卡拉马钱德拉医学中心收集了2011年4月至2013年3月期间获得的70株临床分离株，通过传统表型方法进行鉴定，并纳入本前瞻性研究中。然后对分离株进行聚合酶链反应（PCR），靶向NTS区域的两个亚重复元件（SREs），即TRS-1和TRS-2。
在两个亚重复位点均观察到菌株特异性多态性。结合TRS-1和TRS-2这两个SREs，共获得了9种不同的菌株。
该结果有力地表明了利用NTS区域扩增来区分临床分离株的可行性。该方法可作为一种工具，用于开展毛癣菌感染的流行病学和基于人群的研究。这将有助于未来对毛癣菌流行病学的探索。
