Hei T K, Geard C R, Osmak R S, Travisano M
Br J Cancer. 1985 Oct;52(4):591-7. doi: 10.1038/bjc.1985.231.
The in vitro cytotoxicity and oncogenic potential of both native and acid leached asbestos fibres were studied using the C3H 10T1/2 cell model. Both native and leached fibres induced a dose-dependent toxicity. At high fibre concentrations, acid leached fibres were significantly less toxic than their untreated counterparts. While asbestos fibres alone do not induce oncogenic transformation at the concentration examined, it was found that both leached and native fibres substantially enhanced the oncogenicity of gamma-irradiation in a more than additive fashion. Although no significant chromosomal aberrations or sister chromatid exchanges (SCE) were found in asbestos treated cultures, a significantly higher number of SCEs was observed in cells treated with both asbestos and radiation compared to cells receiving radiation alone. The results suggest that the enhancement in radiation induced oncogenicity by asbestos fibres may be attributed to the mere physical presence of the fibres rather than any chemical contaminants the fibres may contain. Furthermore, the carcinogenicity of asbestos may be unrelated to genotoxicity.
使用C3H 10T1/2细胞模型研究了天然和酸浸石棉纤维的体外细胞毒性和致癌潜力。天然纤维和浸出纤维均诱导了剂量依赖性毒性。在高纤维浓度下，酸浸纤维的毒性明显低于未处理的对应物。虽然单独的石棉纤维在所检测的浓度下不会诱导致癌转化，但发现浸出纤维和天然纤维均以超过相加的方式显著增强了γ射线的致癌性。尽管在石棉处理的培养物中未发现明显的染色体畸变或姐妹染色单体交换（SCE），但与仅接受辐射的细胞相比，在用石棉和辐射处理的细胞中观察到的SCE数量明显更高。结果表明，石棉纤维对辐射诱导致癌性的增强可能归因于纤维的单纯物理存在，而不是纤维可能含有的任何化学污染物。此外，石棉的致癌性可能与遗传毒性无关。
