Morgan D J, Moodley I, Cundell D R, Sheinman B D, Smart W, Davies R J
Clin Sci (Lond). 1985 Jul;69(1):63-9. doi: 10.1042/cs0690063.
Plasma histamine and serum neutrophil chemotactic activity (S-NCA) were measured in ten atopic asthmatic patients on four separate occasions after allergen bronchial provocation testing (BPT). Single doses of inhaled sodium cromoglycate (SCG; 20 mg), clemastine (0.5 mg), ketotifen (0.5 mg) and isotonic saline (0.9% NaCl) placebo were administered 30 min before bronchial provocation testing in random order and double-blind. The airflow obstruction after BPT was monitored by measurement of forced expiratory volume in 1 s (FEV1). Plasma histamine was measured by the double-isotope radioenzymatic assay and S-NCA by a modified Boyden chamber technique. A highly significant decrease in FEV1 after BPT occurred on the placebo pre-treatment visit (P less than 0.001). Prior administration of inhaled SCG, clemastine and ketotifen significantly reduced the decrease in airflow obstruction seen after BPT when compared with placebo treatment (P less than 0.01, P less than 0.02, P less than 0.05 respectively). No significant alteration in plasma histamine was detected during allergen-induced airflow obstruction. Levels of S-NCA were significantly higher 5, 10 and 15 min after BPT when compared with the pre-challenge level (P less than 0.01, P less than 0.01, P less than 0.001 respectively). These levels were not significantly decreased when airflow obstruction was inhibited by the prior inhalation of SCG, clemastine or ketotifen.
在10名特应性哮喘患者进行变应原支气管激发试验（BPT）后的4个不同时间点，测量其血浆组胺和血清中性粒细胞趋化活性（S-NCA）。在支气管激发试验前30分钟，随机、双盲给予单剂量吸入性色甘酸钠（SCG；20毫克）、氯马斯汀（0.5毫克）、酮替芬（0.5毫克）和等渗盐水（0.9%氯化钠）安慰剂。通过测量1秒用力呼气量（FEV1）监测BPT后的气流阻塞情况。采用双同位素放射酶法测量血浆组胺，采用改良的博伊登小室技术测量S-NCA。在安慰剂预处理访视时，BPT后FEV1出现极显著下降（P<0.001）。与安慰剂治疗相比，预先吸入SCG、氯马斯汀和酮替芬可显著减轻BPT后出现的气流阻塞下降（分别为P<0.01、P<0.02、P<0.05）。在变应原诱导的气流阻塞期间，未检测到血浆组胺有显著变化。与激发前水平相比，BPT后5、10和15分钟时S-NCA水平显著升高（分别为P<0.01、P<0.01、P<0.001）。预先吸入SCG、氯马斯汀或酮替芬抑制气流阻塞时，这些水平未显著降低。
