Department of Internal Medicine, Clinical Hospital Centre Rijeka, Kresimirova 42, 51000, Rijeka, Croatia.
Department of Anatomy, Faculty of Medicine, University of Rijeka, Brace Branchetta 20, 51000, Rijeka, Croatia.
J Endocrinol Invest. 2018 Feb;41(2):203-209. doi: 10.1007/s40618-017-0720-0. Epub 2017 Jun 28.
Increased oxidative stress and impaired antioxidant defense are important mechanisms in the pathogenesis of diabetic myopathy. Since diabetes mellitus type 1 decreases muscle regeneration capacity the present study was designed to determine the influence of alpha-lipoic acid (ALA), a potent biological antioxidant, on the process of regeneration of diabetic rat skeletal muscles.
40 Wistar rats were divided into three groups: control (n = 8), untreated diabetic group (n = 16) and ALA treated diabetic group (n = 16). The regeneration process was provoked in streptozotocin-induced diabetic rats in both slow (m.soleus, SOL) and fast (m.extensor digitorum longus, EDL) skeletal muscles by intramuscular injection of myotoxin bupivacaine. At intervals of 10 days and 4 weeks, muscle histochemical and morphometrical analysis (fiber cross areas and fiber type distribution) was performed.
Changes induced by diabetes are evident in redistribution of muscle fibers and in significant level of atrophy. After 4 weeks of diabetes, glycolytic muscle fibers are dominant in both slow and fast muscles. Muscle atrophy is present in all fiber types except in type I of slow skeletal muscle. Treatment with ALA reduce changes in the morphological properties caused by diabetes mellitus type 1 in slow and fast rat skeletal muscles during the process of regeneration.
Treatment with lipoic acid during 4 weeks has shown effects on the redistribution of muscle fibers, and can prevent atrophy in slow and fast diabetic muscle.
氧化应激增加和抗氧化防御受损是糖尿病性肌病发病机制中的重要机制。由于 1 型糖尿病会降低肌肉再生能力，因此本研究旨在确定α-硫辛酸（ALA）作为一种有效的生物抗氧化剂，对糖尿病大鼠骨骼肌再生过程的影响。
将 40 只 Wistar 大鼠分为三组：对照组（n=8）、未治疗的糖尿病组（n=16）和 ALA 治疗的糖尿病组（n=16）。通过肌肉内注射肉毒素布比卡因，在链脲佐菌素诱导的糖尿病大鼠中引发再生过程，涉及慢肌（m.soleus，SOL）和快肌（m.extensor digitorum longus，EDL）骨骼肌。在 10 天和 4 周的间隔时间内，进行肌肉组织化学和形态计量学分析（纤维横截面积和纤维类型分布）。
糖尿病引起的变化表现在肌肉纤维的重新分布和明显的萎缩程度上。糖尿病 4 周后，两种快肌和慢肌中均以糖酵解肌纤维为主。除慢肌的 I 型纤维外，所有纤维类型均存在肌肉萎缩。在慢肌和快肌的再生过程中，用 ALA 治疗可减少 1 型糖尿病引起的形态学特性变化。
在 4 周的治疗中，使用硫辛酸治疗显示出对肌肉纤维再分布的影响，并可预防慢肌和快肌的糖尿病性萎缩。
