Strugala G J, Overhoff H, Forth W
Digestion. 1985;32(4):255-66. doi: 10.1159/000199246.
The transfer and tissue content of 3H-pteroylmonoglutamate (PteGlu) from the mucosal to the serosal side (Jms, TCm) and in the reverse direction (Jsm, TCs) was studied using the everted sac technique. In the entire intestine, except for the colon, 3H-PteGlu was transferred preferentially into the serosal solution. When 3H-PteGlu was applied to the serosal side the final tissue concentration in either jejunal, duodenal, ileal or colonic segments was not significantly different from each other and about two-fold the serosal concentration. Apparently there exists a specific transfer process from the mucosal to the serosal side in the jejunum. The transfer of 3H-PteGlu shows saturation kinetics (S0.5 = 4.9 X 10(-5) mol/l). At low concentration (2 nmol/l) 3H-PteGlu was accumulated within the mucosal epithelium (tissue/mucosal fluid ratio = 3.8). Transfer and accumulation in the mucosal tissue of 3H-PteGlu apparently need high activation energy as indicated by the temperature dependency of these processes. Finally, transfer and accumulation in the tissue of 3H-PteGlu could be inhibited by salazosulfapyridine and phenobarbital.
采用外翻肠囊技术研究了3H-蝶酰单谷氨酸（PteGlu）从黏膜侧到浆膜侧（Jms，TCm）以及反向（Jsm，TCs）的转运和组织含量。在整个肠道中，除结肠外，3H-PteGlu优先转运至浆膜溶液中。当将3H-PteGlu应用于浆膜侧时，空肠、十二指肠、回肠或结肠段的最终组织浓度彼此之间无显著差异，约为浆膜浓度的两倍。显然，空肠中存在从黏膜侧到浆膜侧的特定转运过程。3H-PteGlu的转运呈现饱和动力学（S0.5 = 4.9×10⁻⁵mol/L）。在低浓度（2 nmol/L）时，3H-PteGlu在黏膜上皮内蓄积（组织/黏膜液比值 = 3.8）。这些过程的温度依赖性表明，3H-PteGlu在黏膜组织中的转运和蓄积显然需要高活化能。最后，柳氮磺胺吡啶和苯巴比妥可抑制3H-PteGlu在组织中的转运和蓄积。
