通过紫外可见光谱对活细菌细胞中的新德里金属β-内酰胺酶-1进行实时活性监测。
Real-time activity monitoring of New Delhi metallo-β-lactamase-1 in living bacterial cells by UV-Vis spectroscopy.
作者信息
Yang Ke-Wu, Zhou Yajun, Ge Ying, Zhang Yuejuan
机构信息
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry and Materials Science, Northwest University, Xi'an 710127, P. R. China.
出版信息
Chem Commun (Camb). 2017 Jul 13;53(57):8014-8017. doi: 10.1039/c7cc02774e.
We report an UV-Vis method for monitoring the hydrolysis of the β-lactam antibiotics inside living bacterial cells. Cell-based studies demonstrated that the hydrolysis of cefazolin was inhibited by three known NDM-1 inhibitors. This approach can be applied to the monitoring of reactions in a complex biological system, for instance in medical testing.
我们报告了一种用于监测活细菌细胞内β-内酰胺抗生素水解的紫外-可见光谱法。基于细胞的研究表明，头孢唑林的水解受到三种已知的NDM-1抑制剂的抑制。这种方法可应用于监测复杂生物系统中的反应，例如在医学检测中。
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