Sharma Vikas, Wani Mohammad Saleem, Singh Vijay, Kaur Kuljit, Gupta Raghbir Chand
Department of Botany, Punjabi University, Patiala, Punjab, 147002, India.
3 Biotech. 2017 Jul;7(3):190. doi: 10.1007/s13205-017-0833-3. Epub 2017 Jun 29.
Trillium govanianum is a temperate forest understory plant species of high value belonging to the family Melanthiaceae. It is endemic to Himalayan region and facing a bottleneck situation due to reckless extractions from its natural strands. In the present study, 21 microsatellite markers were developed and characterized in 20 accessions of T. govanianum. Collectively, the polymorphic markers amplified 31 alleles in a range of 2-4 with an average of 2.6 alleles per marker. The mean observed heterozygosity (H ), expected heterozygosity (H ), and Shannon information index (I) were 0.46, 0.48, and 0.73, respectively. Average polymorphism information content (PIC) was 0.385. The cross-transferability in a related species, namely, Polygonatum verticillatum, showed amplification of ten markers. The newly developed microsatellite markers efficiently distinguished the different accessions on the basis of their geographic origin. Thus, these microsatellites can be useful in exploring genetic diversity in various existing populations of T. govanianum in north-western Himalaya, which may be useful for their conservation, management, and improvement in future.
延龄草是延龄草科一种具有高价值的温带森林林下植物物种。它是喜马拉雅地区的特有物种，由于对其天然生长地的肆意采挖而面临瓶颈状况。在本研究中，开发了21个微卫星标记，并在20份延龄草材料中进行了特征分析。总体而言，多态性标记共扩增出31个等位基因，每个标记的等位基因数在2 - 4个之间，平均每个标记2.6个等位基因。观察到的平均杂合度（H ）、期望杂合度（H ）和香农信息指数（I）分别为0.46、0.48和0.73。平均多态性信息含量（PIC）为0.385。在相关物种黄精中交叉转移性研究显示，有10个标记能够扩增。新开发的微卫星标记能够根据地理来源有效区分不同的材料。因此，这些微卫星标记可用于探索喜马拉雅西北部延龄草各种现有种群的遗传多样性，这可能对其未来的保护、管理和改良有用。
