Department of Biochemistry and Molecular Pharmacology, Programs in Neuroscience and Chemical Biology, University of Massachusetts Medical School , 364 Plantation Street, Worcester, Massachusetts 01605, United States.
Department of Natural Sciences, Assumption College , 500 Salisbury Street, Worcester, Massachusetts 01609, United States.
J Org Chem. 2017 Aug 4;82(15):8199-8205. doi: 10.1021/acs.joc.7b00845. Epub 2017 Jul 14.
Potassium (K) exits electrically excitable cells during normal and pathophysiological activity. Currently, K-sensitive electrodes and electrical measurements are the primary tools to detect K fluxes. Here, we describe the synthesis of a near-IR, oxazine fluorescent K sensor (K-1) with a dissociation constant suited for detecting changes in intracellular and extracellular K concentrations. K-1 treatment of cells expressing voltage-gated K channels enabled the visualization of intracellular K depletion upon channel opening and restoration of cytoplasmic K after channel closing.
钾（K）在正常和病理生理活动期间从电兴奋细胞中逸出。目前，K 敏感电极和电测量是检测 K 通量的主要工具。在这里，我们描述了一种近红外，吖嗪荧光 K 传感器（K-1）的合成，其离解常数适合检测细胞内和细胞外 K 浓度的变化。用 K-1 处理表达电压门控 K 通道的细胞，可在通道开放时观察到细胞内 K 耗竭，并在通道关闭后细胞质 K 恢复。
