Luo Weifang, Li Jing, Liu Weisheng
Key Laboratory of Nonferrous Metals Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China.
Org Biomol Chem. 2017 Jul 21;15(27):5846-5850. doi: 10.1039/c7ob01189j. Epub 2017 Jun 30.
A novel ratiometric ESIPT fluorescent probe, which is specific for palladium species of all the typical oxidation states (0, +2, and +4), has been designed. Notably, based on the excited state intramolecular proton transfer (ESIPT) process, the probe exhibits a ratiometric fluorescent response to palladium species with a low detection limit (9.0 nM, 0.96 ppb) in about 1.5 min at room temperature. Moreover, it has been successfully used as a two-photon ratiometric fluorescent palladium probe for in vivo and three-dimensional imaging with low cytotoxicity and autofluorescence. Compared with other reported palladium probes, the probe displays a shorter ratiometric response time and lower detection limit in milder test conditions. All of the results indicate that the probe may be favorable for environmental and biological applications.
设计了一种新型的比率型激发态分子内质子转移（ESIPT）荧光探针，它对所有典型氧化态（0、+2和+4）的钯物种具有特异性。值得注意的是，基于激发态分子内质子转移（ESIPT）过程，该探针在室温下约1.5分钟内对钯物种表现出比率型荧光响应，检测限低（9.0 nM，0.96 ppb）。此外，它已成功用作双光子比率型荧光钯探针，用于体内和三维成像，具有低细胞毒性和自发荧光。与其他报道的钯探针相比，该探针在更温和的测试条件下显示出更短的比率响应时间和更低的检测限。所有结果表明，该探针可能有利于环境和生物应用。
