Li Yan, Zhou Mingyang, Wang Fengqing, Wang En Tao, Du Zongjun, Wu Chaoya, Zhang Zhenpeng, Liu Wei, Xie Zhihong
Key Laboratory of Coastal Biology and Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China.
School of Chemistry and Pharmaceutical Engineering, Qilu University of Technology, Jinan 250353, PR China.
Int J Syst Evol Microbiol. 2017 Jun;67(6):1835-1840. doi: 10.1099/ijsem.0.001873.
A protease-producing bacterial strain, 13-12T, was isolated from the ocean sediment of Laizhou Bay, PR China and systematically studied. The bacterium was Gram-stain negative, non spore-forming rods, which were motile with two flagella. It was positive for oxidase, the hydrolysis of starch, agar and gelatin, and for nitrate reduction. It was negative for catalase, esterase and the degradation of CM-cellulose. Optimum growth was observed at 28 °C, pH 6.5-7.0 and in the presence of 2-3 % (w/v) NaCl. Phylogenetic analysis of the 16S rRNA gene, and whole genome data, affiliated it to the genus Photobacterium. It was most closely related to Photobacterium jeanii R-40508T (96.7 % 16S rRNA gene similarity). Strain 13-12T was found to have less than 86.1 % similarities with the type strains of its most closely related species in multi-locus sequence analysis, less than 75.2 % using genome average nucleotide identities (ANI), and less than 18.5 % in DNA-DNA relatedness studies. Q8 was the predominant respiratory menaquinone. Phosphatidylethanolamine, phosphoaminolipid and phospholipid were the major polar phospholipids and summed feature 3 (48.2 %), C16 : 0 (18.4 %) and C18 : 1ω5c (14.1 %) the major fatty acids. The combined phenotypic, phylogenetic, genomic and chemotaxonomic data support this strain representing a novel species of the genus Photobacterium, for which the name Photobacterium proteolyticum sp. nov. is proposed, with 13-12T (=KCTC 42764T=CGMCC 1.14970) as the type strain. The genome size of 13-12T is 6.2 Mbp, comprising 5806 predicted genes and the DNA G+C content is 47.9 mol%.
从中国莱州湾海洋沉积物中分离出一株产蛋白酶的细菌菌株13 - 12T，并对其进行了系统研究。该细菌革兰氏染色阴性，为无芽孢杆菌，具两根鞭毛，能运动。氧化酶、淀粉水解、琼脂水解、明胶水解及硝酸盐还原试验呈阳性。过氧化氢酶、酯酶及CM - 纤维素降解试验呈阴性。在28 °C、pH 6.5 - 7.0及2 - 3 %（w/v）NaCl存在的条件下观察到最佳生长状态。对16S rRNA基因及全基因组数据进行系统发育分析，结果表明它属于发光杆菌属。它与琼氏发光杆菌R - 40508T关系最为密切（16S rRNA基因相似性为96.7 %）。在多位点序列分析中，菌株13 - 12T与其关系最密切物种的模式菌株的相似性低于86.1 %，使用基因组平均核苷酸同一性（ANI）时低于75.2 %，在DNA - DNA相关性研究中低于18.5 %。Q8是主要的呼吸醌。磷脂酰乙醇胺、磷酸氨基脂质和磷脂是主要的极性磷脂，主要脂肪酸为总和特征3（48.2 %）、C16 : 0（18.4 %）和C18 : 1ω5c（14.1 %）。综合表型、系统发育、基因组和化学分类学数据支持该菌株代表发光杆菌属的一个新物种，为此提出新名称解蛋白发光杆菌（Photobacterium proteolyticum sp. nov.），模式菌株为13 - 12T（=KCTC 42764T=CGMCC 1.14970）。13 - 12T的基因组大小为6.2 Mbp，包含5806个预测基因，DNA G + C含量为47.9 mol%。
