Department of Thyroid and Breast Surgery, Ningbo Yinzhou No. 2 Hospital, Ningbo 315040, P.R. China
Department of Thyroid and Breast Surgery, Ningbo Yinzhou No. 2 Hospital, Ningbo 315040, P.R. China.
Biosci Rep. 2017 Aug 2;37(4). doi: 10.1042/BSR20170121. Print 2017 Aug 31.
The present study explores the correlation of human epidermal growth factor receptor-2 (HER-2) protein expression with sentinel lymph node (SLN) metastasis and prognosis of breast cancer. The breast cancer tissues and adjacent tissues were obtained from patients with primary breast cancer. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the mRNA level of HER-2. Spearman correlation analysis was used to analyze the correlation of HER-2 expression with SLN metastasis. The disease-free survival (DFS) and overall survival (OS) of breast cancer patients were investigated. Univariate and multivariate analyses were performed to explore factors influencing SLN metastasis and prognosis of breast cancer. Compared with adjacent tissues, HER-2 expression was significantly up-regulated in breast cancer tissues. HER-2 expression was correlated with the pathological type, tumor node metastasis (TNM) staging, histological grade, blood vessel invasion, SLN metastasis, estrogen receptor (ER), and progesterone receptor (PR). The expression level of HER-2 was positively related to the SLN metastasis (=0.548). Median DFS and OS were longer in patients with negative HER-2 expression than in patients with positive HER-2 expression. TNM staging, SLN metastasis, and expression levels of HER-2 and ER were independent factors for DFS of breast cancer patients, while TNM staging, blood vessel invasion, histological grade, SLN metastasis, and expression levels of HER-2 and PR were independent factors for OS of breast cancer patients. Our study suggests that high expression of HER-2 promoted SLN metastasis. HER-2 expression and SLN metastasis were the independent factors for the prognosis of breast cancer.
本研究探讨了人表皮生长因子受体-2（HER-2）蛋白表达与乳腺癌前哨淋巴结（SLN）转移和预后的相关性。收集原发性乳腺癌患者的乳腺癌组织及相邻组织，采用实时荧光定量聚合酶链反应（qRT-PCR）检测 HER-2mRNA 水平，Spearman 相关分析 HER-2 表达与 SLN 转移的相关性，分析乳腺癌患者无病生存（DFS）和总生存（OS）情况，采用单因素和多因素分析探讨影响 SLN 转移和乳腺癌预后的因素。与相邻组织相比，乳腺癌组织中 HER-2 表达明显上调。HER-2 表达与病理类型、肿瘤淋巴结转移（TNM）分期、组织学分级、血管浸润、SLN 转移、雌激素受体（ER）和孕激素受体（PR）有关。HER-2 表达与 SLN 转移呈正相关（=0.548）。HER-2 表达阴性患者的 DFS 和 OS 均长于 HER-2 表达阳性患者。TNM 分期、SLN 转移及 HER-2、ER 表达水平是乳腺癌患者 DFS 的独立影响因素，而 TNM 分期、血管浸润、组织学分级、SLN 转移及 HER-2、PR 表达水平是乳腺癌患者 OS 的独立影响因素。本研究提示 HER-2 高表达促进 SLN 转移，HER-2 表达及 SLN 转移是乳腺癌患者预后的独立影响因素。
